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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 57 (1999), S. 71-73 
    ISSN: 1573-5044
    Keywords: liana ; micropropagation ; naphthylisoquinoline alkaloids ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae), a West African liana producing naphthylisoquinoline alkaloids, was successfully raised from seeds in vitro. Clonal propagation was best achieved growing nodal stem segments on 1/5 Linsmaier and Skoog medium with full strength organics and supplemented with 0.02 μM thidiazuron, 4.44 μM 6-benzylaminopurine and 0.05 μM 1-naphthaleneacetic acid. Detached axillary shoots were grown on Anderson's Rhododendron medium devoid of phytohormones and rooted within one month when dipped in 4.92 μM indole-3-butyric acid. Rooted plants became acclimatized to nonsterile greenhouse conditions.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Nuclear magnetic resonance micro-imaging ; Chemical-shift imaging ; Fluorescence microscopy ; Ancistrocladus heyneanus ; Ancistrocladaceae ; Naphthylisoquinoline alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The tropical lianaAncistrocladus heyneanus, which is known for its biologically active naphthylisoquinoline alkaloids, has been studied by nuclear magnetic resonance (NMR) microscopy for the first time. The spatial resolution of the cross-sectional NMR images was of the order of 20 μm. Quantitative NMR relaxation time images of the root and the shoot show great contrast between different tissue regions. In addition, we observed the regional distribution of chemical compounds inAncistrocladus heyneanus by chemical-shift NMR microscopy. The NMR imaging results were compared with light and fluorescence microscopic images and reveal the excellent tissue characterization using NMR technology.
    Type of Medium: Electronic Resource
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