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In vitro propagation of Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae)

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Abstract

Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae), a West African liana producing naphthylisoquinoline alkaloids, was successfully raised from seeds in vitro. Clonal propagation was best achieved growing nodal stem segments on 1/5 Linsmaier and Skoog medium with full strength organics and supplemented with 0.02 μM thidiazuron, 4.44 μM 6-benzylaminopurine and 0.05 μM 1-naphthaleneacetic acid. Detached axillary shoots were grown on Anderson's Rhododendron medium devoid of phytohormones and rooted within one month when dipped in 4.92 μM indole-3-butyric acid. Rooted plants became acclimatized to nonsterile greenhouse conditions.

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Authors and Affiliations

  1. Institut für Organische Chemie der Universität, Am Hubland, D-97074, Würzburg, Germany

    G. Bringmann, H. Rischer & J. Schlauer

  2. Centre National de Floristique, Université d'Abidjan, 08 B.P. 172, Abidjan 08, Ivory Coast

    L. Aké Assi

Authors
  1. G. Bringmann
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  2. H. Rischer
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  3. J. Schlauer
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  4. L. Aké Assi
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Correspondence to G. Bringmann.

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Bringmann, G., Rischer, H., Schlauer, J. et al. In vitro propagation of Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae). Plant Cell, Tissue and Organ Culture 57, 71–73 (1999). https://doi.org/10.1023/A:1006201711390

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  • DOI: https://doi.org/10.1023/A:1006201711390

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