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  • Springer  (21)
  • National Academy of Sciences
  • 1995-1999  (21)
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Year
  • 1
    ISSN: 1432-1432
    Keywords: Key words:Squalus acanthias— Carbamoyl-phosphate synthetase — Promoter —Rana catesbeiana— TATA box — TACAAA — C/EBP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Carbamoyl-phosphate synthetase III (CPSase III) of Squalus acanthias (spiny dogfish) is a nuclear-encoded mitochondrial enzyme that catalyzes glutamine-dependent formation of carbamoyl phosphate for urea synthesis. In this paper we report the results of cloning a 10-kb segment of genomic DNA which includes the region flanking the 5′ end of the spiny dogfish CPSase III gene. A total of 1,295 base pairs of sequence straddling the start codon was obtained. Primer extension experiments revealed that the transcription start site is the G located 114 residues upstream of the translation start codon ATG. The first exon has 240 base pairs, including the 5′ untranslated region, the coding sequence for the signal peptide (38 amino acids), and the four N-terminal amino acids of the mature enzyme. The boundary of the first exon and the first intron of the CPSase III gene is concordant with that of rat and frog (Rana catesbeiana) CPSase I, which have been suggested to have evolved from CPSase III. The putative TATA box sequence, TACAAA, is located at position −31 with an uncommonly found C at the third position. Two C/EBP binding site sequences, ATTCTGCAAG (−405 to −397) and GTGCAGTAAG (−168 to −160), were identified in the promoter region, which suggests that spiny dogfish CPSase III might be subjected to transactivation of transcription by C/EBP-related proteins, as has been reported for rat CPSase I. The preparation and binding of a recombinant RcC/EBP-1 protein (the R. catesbeiana homolog of the mammalian C/EBPα) to the two spiny dogfish C/EBP binding sequences are described. Two putative heat-shock binding elements were also identified in the promoter region.
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  • 2
    ISSN: 1432-1432
    Keywords: Squalus acanthias ; Carbamoyl-phosphate synthetase ; Promoter ; Rana catesbeiana ; TATA box ; TACAAA ; C/EBP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Carbamoyl-phosphate synthetase III (CPSase III) ofSqualus acanthias (spiny dogfish) is a nuclear-encoded mitochondrial enzyme that catalyzes glutamine-dependent formation of carbamoyl phosphate for urea synthesis. In this paper we report the results of cloning a 10-kb segment of genomic DNA which includes the region flanking the 5′ end of the spiny dogfish CPSase III gene. A total of 1,295 base pairs of sequence straddling the start codon was obtained. Primer extension experiments revealed that the transcription start site is the G located 114 residues upstream of the translation start codon ATG. The first exon has 240 base pairs, including the 5′ untranslated region, the coding sequence for the signal peptide (38 amino acids), and the four N-terminal amino acids of the mature enzyme. The boundary of the first exon and the first intron of the CPSase III gene is concordant with that of rat and frog (Rana catesbeiana) CPSase I, which have been suggested to have evolved from CPSase III. The putative TATA box sequence, TACAAA, is located at position −31 with an uncommonly found C at the third position. Two C/EBP binding site sequences, ATTCTGCAAG (−405 to −397) and GTGCAGTAAG (−168 to −160), were identified in the promoter region, which suggests that spiny dogfish CPSase III might be subjected to transactivation of transcription by C/EBP-related proteins, as has been reported for rat CPSase I. The preparation and binding of a recombinant RcC/EBP-1 protein (theR. catesbeiana homolog of the mammalian C/EBPα) to the two spiny dogfish C/EBP binding sequences are described. Two putative heatshock binding elements were also identified in the promoter region.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 252 (1996), S. 33-41 
    ISSN: 1617-4623
    Keywords: Teosinte ; Maize ; Morphological traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The segregation of key traits distinguishing maize and teosinte was analyzed in three F2 and three backcross populations derived from crosses of the modern maize inbred T232 withZea mays ssp.parviglumis. These traits were (i) paired vs. single female spikelets; (ii) two-ranked vs. many-ranked ears; (iii) non-indurated vs. indurated glumes; (iv) inclination of the kernels toward the rachis, and (v) distichous vs. polystichous central staminate spike. All traits showed a simple mode of inheritance except for paired female spikes, which appeared to be controlled by two genes. The loci controlling these major changes were mapped with RFLP markers to four chromosomal regions. These results support the suggestion that maize became differentiated from teosinte with as few as five major gene changes.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 252 (1996), S. 33-41 
    ISSN: 1617-4623
    Keywords: Key words Teosinte ; Maize ; Morphological traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The segregation of key traits distinguishing maize and teosinte was analyzed in three F2 and three backcross populations derived from crosses of the modern maize inbred T232 with Zea mays ssp. parviglumis. These traits were (i) paired vs. single female spikelets; (ii) two-ranked vs. many-ranked ears; (iii) non-indurated vs. indurated glumes; (iv) inclination of the kernels toward the rachis, and (v) distichous vs. polystichous central staminate spike. All traits showed a simple mode of inheritance except for paired female spikes, which appeared to be controlled by two genes. The loci controlling these major changes were mapped with RFLP markers to four chromosomal regions. These results support the suggestion that maize became differentiated from teosinte with as few as five major gene changes.
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  • 5
    ISSN: 1436-2236
    Keywords: Key words:Sebastes, rockfish, microsatellites.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: We describe the first microsatellites for rockfishes in the diverse genus Sebastes. Clones containing microsatellites were isolated from the genomic library of a quillback rockfish, Sebastes maliger. Twelve microsatellites are characterized; six of these are polymorphic in quillback rockfish, and eight are polymorphic in at least one rockfish species on which they were tested. The number of alleles per variable locus ranged from 4 to 15 and averaged 6.8. The expected heterozygosities ranged from 0.38 to 0.79 and averaged 0.60 in these loci. These loci should prove valuable in studies examining species identification, population genetics, hybridization, paternity, kinship, and microsatellite evolution.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archive for mathematical logic 37 (1998), S. 199-214 
    ISSN: 1432-0665
    Keywords: Mathematics Subject Classification (1991): 03F10, 03F25
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract. The subject of this paper is a characterization of the $\Sigma_1$ -definable set functions of Kripke-Platek set theory with infinity and a uniform version of axiom of choice: $KP\omega+(uniform\;AC)$ . This class of functions is shown to coincide with the collection of set functionals of type 1 primitive recursive in a given choice functional and $x\mapsto\omega$ . This goal is achieved by a Gödel Dialectica-style functional interpretation of $KP\omega+(uniform\;AC)$ and a computability proof for the involved functionals.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archive of applied mechanics 65 (1995), S. 437-456 
    ISSN: 1432-0681
    Keywords: continuum damage ; micro-mechanics ; ceramic matrix ; composite modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary Continuum Damage Mechanics has been applied successfully to technical problems since the idea was introduced by Kachanov almost 40 years ago. In keeping with the traditions of mechanics, the formulation was based on the results of mechanical tests on specimens whose size is measured in centimeters. To model the observations which describe the deterioration of material properties it was found necessary to introduce internal variables referred to as ‘damage’. The approach is phenomenological, with only a minimal attempt to provide a physical interpretation of damage. For this reason the approach has had little appeal to those whose interest is in the physical mechanisms which cause material deterioration. In this presentation a description is given of attempts to develop continuum damage mechanics so that the relationship with the physical mechanism approach is less abrupt. The procedure is illustrated with reference to ceramic matrix composites.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archive of applied mechanics 65 (1995), S. 437-456 
    ISSN: 1432-0681
    Keywords: Key words continuum damage ; micro-mechanics ; ceramic matrix ; composite modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary  Continuum Damage Mechanics has been applied successfully to technical problems since the idea was introduced by Kachanov almost 40 years ago. In keeping with the traditions of mechanics, the formulation was based on the results of mechanical tests on specimens whose size is measured in centimeters. To model the observations which describe the deterioration of material properties it was found necessary to introduce internal variables referred to as ‘damage’. The approach is phenomenological, with only a minimal attempt to provide a physical interpretation of damage. For this reason the approach has had little appeal to those whose interest is in the physical mechanisms which cause material deterioration. In this presentation a description is given of attempts to develop continuum damage mechanics so that the relationship with the physical mechanism approach is less abrupt. The procedure is illustrated with reference to ceramic matrix composites.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Wireless personal communications 10 (1999), S. 3-17 
    ISSN: 1572-834X
    Keywords: OFDM ; coding ; broadband ; multipath
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract This paper gives a unified analysis of the effects of multipath dispersion and Doppler spreading due to fast fading in OFDM systems, deriving simple closed-form expressions for intersymbol and interchannel interference in the most important cases. It shows also why sine wave carriers are in general the optimum bearers for the sub-channels. The expressions for interference are used to estimate the BER performance of coded OFDM, showing that the use of forward error correction (FEC) coding and a guard period are both essential for optimum performance. As an example it is shown that a 64 sub- channel OFDM system can achieve a data rate of 6.4 Mbit/s on a typical urban mobile radio channel.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Environmental biology of fishes 47 (1996), S. 142-142 
    ISSN: 1573-5133
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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