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  • Springer  (85)
  • 2000-2004  (32)
  • 1995-1999  (53)
  • 1
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    A perfusion-cell bleeding culture strategy for enhancing the productivity of eicosapentaenoic acid by Nitzschia laevis (2001)
    Springer
    Details
    Publication Date: 2001-10-01
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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    PAPER CURRENT
  • 2
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    Geochemical and isotopic composition and inherited zircon ages as evidence for lower crustal origin of two Variscan S-type granites in the NW Bohemian massif (2003)
    Springer
    Details
    Publication Date: 2003-03-20
    Print ISSN: 1437-3254
    Electronic ISSN: 1437-3262
    Topics: Geosciences
    Published by Springer
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    PAPER CURRENT
  • 3
    Electronic Resource
    Electronic Resource
    Cloning and expression analysis of murine lupin, a member of a novel gene family that is conserved through evolution and associated with Lupus inclusions (2000)
    Springer
    Development genes and evolution 210 (2000), S. 512-517 
    Details
    ISSN: 1432-041X
    Keywords: Key words Systemic lupus erythematosis ; AIDS ; HIV ; p36 ; Cytoplasmic inclusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe here the first full-length sequence of a member of a novel gene family encoding a protein in the mouse that we call Lupin. Lupin is homologous to a human protein previously called p36, which was purified from α-interferon-treated cells that formed lupus inclusions. Lupus inclusions are dense intracellular deposits found in endothelial cells and lymphocytes of patients with systemic lupus erythematosis and AIDS. Proteins closely related to Lupin exist in evolutionarily divergent species including Caenorhabditis elegans, Drosophila and zebrafish. At least one other lupin-related gene is expressed in the mouse and in man. Lupin is expressed in mouse embryos and adults, notably in liver, spleen, central nervous system, multiple epithelia and all types of muscle. In skeletal muscle, expression analysis suggests that Lupin associates with the contractile apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270000093
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  • 4
    Electronic Resource
    Electronic Resource
    Subitaneous, diapause, and delayed-hatching eggs of planktonic copepods from the northern Gulf of Mexico: morphology and hatching success (1997)
    Springer
    Marine biology 127 (1997), S. 587-597 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experiments were conducted to examine the morphology and hatching success of eggs, either spawned by freshly caught planktonic copepods or recovered from bottom sediments in the northern Gulf of Mexico. Collections were made between August 1992 and September 1995. Eggs of nine species were described and these differed in their diameter, color and surface attributes. Three types of eggs were distinguished: subitaneous, diapause, and delayed-hatching. Three species, Labidocera aestiva Wheeler, Acartia tonsa Dana, and Centropages velificatus (Oliveira) produced only subitaneous eggs. Hatching success varied greatly among these species. Two species, Labidocera mirabilis Fleminger and Centropages hamatus (Lilljeborg) produced diapause eggs and subitaneous eggs. The length of the refractory phase of the diapause eggs differed greatly both within and between these two species. A third type of dormant egg, delayed-hatching, was recognized in Labidocera scotti Giesbrecht and Pontella meadi Wheeler. The existence of delayed-hatching eggs may be an adaptive response of subtropical species to less seasonal fluctuation. Based upon morphological characteristics of the eggs and rearing of nauplii to an identifiable stage, benthic dormant eggs of eight species of calanoid copepods were also identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050049
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  • 5
    Electronic Resource
    Electronic Resource
    Geochemical exploration for platinum-group elements in the Bushveld Complex, South Africa (1997)
    Springer
    Mineralium deposita 32 (1997), S. 349-361 
    Details
    ISSN: 1432-1866
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Analyses of stream sediment and soil samples from the Bushveld Complex, South Africa have revealed enhanced precious metal concentrations, which can be related both to mining activities and the presence of hidden concentrations of platinum-group elements (PGEs) and gold. The economically important PGE deposits hosted by the Upper Critical Zone of the Rustenburg Layered Suite are revealed by a high PGE and Au content in the overlying soils. A second zone of elevated precious metal concentrations straddles the boundary between the Main and Upper Zones and has to date been traced for more than 100 km. This zone follows the igneous layering of the Rustenburg Layered Suite and is offset by the Brits Graben. It is therefore thought to be the reflection of a magmatic PGE-Au mineralisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001260050101
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  • 6
    Electronic Resource
    Electronic Resource
    A new method to normalize the effect of matrix properties on the value of interfacial shear strength obtained from the fragmentation test (1997)
    Springer
    Journal of materials science 32 (1997), S. 4759-4765 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A new method, based on tensile yield strength and strain, has been developed to normalize the effect of matrix properties on the critical fibre length and the interfacial shear strength obtained from the fragmentation test. It is argued that the conventional data normalization technique which employs elastic properties of the matrix, is fundamentally flawed because the model employed to calculate interfacial shear strength assumes perfect plasticity. Single embedded fibre fragmentation in a range of epoxy resins with differing mechanical properties has been used to validate the new method. Stoichiometric quantities of the current agent were used to keep the same interfacial chemistry. The proposed method provides more consistent interfacial shear strength data than existing theories. Furthermore, this normalization technique can also be used to predict the interfacial shear strength of glass fibres embedded in a range of support resins, such as vinyl ester or epoxy resins. For these cases, a thin layer of the phenolic resin was used on the glass fibre to keep the interface chemistry the same.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018631030753
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  • 7
    Electronic Resource
    Electronic Resource
    Production of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhiza (1999)
    Springer
    Journal of industrial microbiology and biotechnology 22 (1999), S. 133-138 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: Salvia miltiorrhiza; Lamiaceae; Agrobacterium rhizogenes; hairy roots; lithospermic acid B; rosmarinic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hairy root cultures of Salvia miltiorrhiza were established by infecting sterile plantlets with Agrobacterium rhizogenes ATCC 15834, and the transformation was proved by direct detection of the inserted T-DNA by the polymerase chain reaction. As determined by HPLC, these hairy root cultures had the ability to produce lithospermic acid B (LAB), rosmarinic acid (RA) and other related phenolic compounds, the water-soluble active components of the plant. The effect of five different basal media, MS, MS-NH〈INF〉4〈/INF〉 (MS without ammonium nitrate), B5, WPM and 6,7-V on the root growth and phenolic compound production was studied. It was found that MS-NH〈INF〉4〈/INF〉 and 6,7-V media were superior to MS, B5 and WPM media in terms of both root growth and phenolic compound production. The time course of biomass accumulation and phenolic compound formation was also examined in the culture using MS-NH〈INF〉4〈/INF〉medium. During cultivation, the content of RA in the roots was stable being approximately 0.48% of dry weight while the content of LAB fluctuated between 0.73% and 1.61% of dry weight, and decreased gradually at the stationary phase of growth. The highest production of LAB and RA was about 64 mg L−1 and 23 mg L−1, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900624
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  • 8
    Electronic Resource
    Electronic Resource
    Kinetic models for astaxanthin production by high cell density mixotrophic culture of the microalga Haematococcus pluvialis (1999)
    Springer
    Journal of industrial microbiology and biotechnology 23 (1999), S. 691-696 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: Haematococcus pluvialis; mixotrophic culture; light irradiance; astaxanthin production; kinetic model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: High cell density cultivation of Haematococcus pluvialis for astaxanthin production was carried out in batch and fed-batch modes in 3.7-L bioreactors with stepwise increased light intensity control mode. A high cell density of 2.65 g L−1 (batch culture) or 2.74 g L−1 (fed-batch culture) was obtained, and total astaxanthin production in the fed-batch culture (64.36 mg L−1) was about 20.5% higher than in the batch culture (53.43 mg L−1). An unstructured kinetic model to describe the microalga culture system including cell growth, astaxanthin formation, as well as sodium acetate consumption was proposed. Good agreement was found between the model predictions and experimental data. The models demonstrated that the optimal light intensity for mixotrophic growth of H. pluvialis in batch or fed-batch cultures in a 3.7-L bioreactor was 90–360 μmol m−2 s−1, and that the stepwise increased light intensity mode could be replaced by a constant light intensity mode.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900685
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  • 9
    Electronic Resource
    Electronic Resource
    Dynamics and stability analysis of the growth and astaxanthin production system of Haematococcus pluvialis (1999)
    Springer
    Journal of industrial microbiology and biotechnology 23 (1999), S. 133-137 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: Haematococcus pluvialis; mixotrophic culture; astaxanthin production; dynamic model; stability analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper investigated high cell density cultivation of Haematococcus pluvialis for astaxanthin production in 3.7-L bioreactors. A biomass concentration of 2.74 g L−1and an astaxanthin yield of 64.4 mg L−1 were obtained. Based on the experimental results, a new and simple dynamic model is proposed, differing from Monod kinetics, to describe cell growth, product formation and substrate consumption. Good agreement was found between the model predictions and experimental data. The model revealed that there was cell growth inhibition on product formation and product feedback compensation for substrate consumption, but no substrate inhibition or product inhibition of cell growth. Stability analysis demonstrated that no multiplicity of steady states was observed; the unique positive steady state was locally asymptotically stable; and the effect of dilution rate on steady states was greater than that of the initial substrate concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900704
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  • 10
    Electronic Resource
    Electronic Resource
    Heterotrophic production of eicosapentaenoid acid by the diatom Nitzschia laevis: effects of silicate and glucose (2000)
    Springer
    Journal of industrial microbiology and biotechnology 25 (2000), S. 218-224 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: diatom; Nitzschia laevis; EPA; glucose; silicate; heterotrophic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of silicate and glucose on growth and eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis were studied. By alternately altering the concentrations of silicate (2.7–64 mg l−1) and glucose (1–40 g l−1) in the medium, the highest cell dry weight (ca. 5.5 g l−1) was obtained at 20 g l−1 glucose and 32 mg l−1 silicate, while the highest specific growth rate (ca. 0.65 day−1) was obtained at a relatively low glucose concentration (5 g l−1) and high silicate concentrations (32–64 mg l−1). At glucose levels of 5 and 20 g l−1, EPA content was higher with lower silicate concentrations (2.7 and 16 mg l−1 silicate, respectively), while at a silicate level of 16 mg l−1, higher glucose concentrations (20–40 g l−1) facilitated EPA formation. The highest EPA yield (131 mg l−1) was obtained at 20 g l−1 glucose and 32 mg l−1 silicate, while the highest EPA productivity (15.1 mg l−1 day−1) was obtained at 20 g l−1 glucose and 64 mg l−1 silicate. Journal of Industrial Microbiology & Biotechnology (2000) 25, 218–224.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.7000056
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