Publication Date:
1995-12-01
Description:
Intracellular calcium (Ca2+) inhibits the opening of L-type (alpha 1C) Ca2+ channels, providing physiological control of Ca2+ entry into a wide variety of cells. A structural determinant of this Ca(2+)-sensitive inactivation was revealed by chimeric Ca2+ channels derived from parental alpha 1C and alpha 1E channels, the latter of which is a neuronal channel lacking Ca2+ inactivation. A consensus Ca(2+)-binding motif (an EF hand), located on the alpha 1C subunit, was required for Ca2+ inactivation. Donation of the alpha 1C EF-hand region to the alpha 1E channel conferred the Ca(2+)-inactivating phenotype. These results strongly suggest that Ca2+ binding to the alpha 1C subunit initiates Ca2+ inactivation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉de Leon, M -- Wang, Y -- Jones, L -- Perez-Reyes, E -- Wei, X -- Soong, T W -- Snutch, T P -- Yue, D T -- New York, N.Y. -- Science. 1995 Dec 1;270(5241):1502-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7491499" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Barium/metabolism
;
Calcium/*metabolism/pharmacology
;
Calcium Channels/chemistry/*metabolism
;
Cell Line
;
Cyclic AMP-Dependent Protein Kinases/metabolism
;
Humans
;
Ion Channel Gating
;
Molecular Sequence Data
;
Patch-Clamp Techniques
;
Phosphorylation
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Sequence Alignment
;
Up-Regulation
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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