Publication Date:
1998-03-07
Description:
Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, S H -- Lin, D P -- Matsumoto, S -- Kitazono, A -- Matsumoto, T -- 5T32GM07491/GM/NIGMS NIH HHS/ -- R01GM56305/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1998 Feb 13;279(5353):1045-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9461438" target="_blank"〉PubMed〈/a〉
Keywords:
Anaphase
;
Anaphase-Promoting Complex-Cyclosome
;
Calcium-Binding Proteins/genetics/*metabolism
;
Carrier Proteins/genetics/*metabolism
;
Cdc20 Proteins
;
*Cell Cycle Proteins
;
Chromosomes, Fungal/metabolism
;
DNA Replication
;
Fungal Proteins/genetics/*metabolism
;
Genes, Fungal
;
Kinesin/genetics/metabolism
;
Ligases/metabolism
;
Mad2 Proteins
;
Metaphase
;
*Mitosis
;
Mutagenesis, Site-Directed
;
Mutation
;
Nuclear Proteins
;
Recombinant Fusion Proteins/metabolism
;
Schizosaccharomyces/*cytology/genetics/*metabolism
;
*Schizosaccharomyces pombe Proteins
;
Spindle Apparatus/*metabolism
;
Transformation, Genetic
;
*Ubiquitin-Protein Ligase Complexes
;
Ubiquitin-Protein Ligases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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