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  • 1
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    The influence of nitrogen and phosphorus on the growth of a diatom Skeletonema costatum (Greville) Cleve (2021)
    In:  http://aquaticcommons.org/id/eprint/16382 | 12051 | 2015-03-27 10:07:19 | 16382 | Bangladesh Fisheries Research Institute
    Details
    Publication Date: 2021-07-04
    Description: Nitrogen and phosphorus requirements of a chain-forming diatom, Skeletonema costatum (Greville) Cleve, collected from Yatsushiro Sea, Japan, were investigated in a laboratory culture experiment. Sodium nitrate and sodium glycerophosphate were used as nitrogen and phosphorus sources, respectively. Cultures were grown in modified Provasoli's ASP2NTA medium (Provasoli et al. 1957) at 25±1°C, light intensity 60 µE mˉ² secˉ¹ and photoperiod 12:12-h, L:D cycle. Optimum growth was observed at nitrate concentrations of 3-10 mglˉ¹ and phosphate concentrations of 1.5-15 mglˉ¹. Adequate growth was also found at the nitrate concentration of up to as high as 300 mglˉ¹. Significantly poorer growth was found at lower nitrate (〈3.0 mglˉ¹) and higher phosphate (〉15 mglˉ¹) concentrations. From the present study, it is concluded that S. costatum can grow well at wide ranges of nitrate concentrations but is sensitive to higher phosphate concentrations.
    Keywords: Aquaculture ; marine algae ; diatom ; Skeletonema costatum ; nitrogen ; phosphorus ; growth rate
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 23-29
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  • 2
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    Physiological observations on a diatom Skeletonema costatum (Greville) Cleve (2021)
    In:  http://aquaticcommons.org/id/eprint/16394 | 12051 | 2015-03-27 09:59:33 | 16394 | Bangladesh Fisheries Research Institute
    Details
    Publication Date: 2021-07-04
    Description: A chain-forming diatom Skeletonema costatum (Greville) Cleve collected from Yatsushiro Sea, Japan was cultured to determine the optimum level of some physico-chemical factors for their growth under laboratory conditions. Filtered and sterilized aged sea water enriched by adding nutrient solution (Provasoli 1968) was used as the culture medium. The plankton could tolerate a wide range of salinities (3-55 ppt). Optimum growth was observed at salinities of 20-35 ppt, temperatures of 20-25°C, light intensities of 80-120µE mˉ² secˉ¹ and pH between 7.5 and 8.0. Growth did not occur at salinities below 3 ppt and at temperatures above 30°C. From the present study, it is concluded that S. costatum was extremely euryhaline and tolerable to very low salinities.
    Keywords: Aquaculture ; diatom ; Skeletonema costatum ; physico-chemical factors ; euryhaline ; laboratory culture ; marine algae salinity tolerance
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 109-118
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  • 3
    Electronic Resource
    Electronic Resource
    Neurotoxins in a toxic red tide of Heterosigma akashiwo (Raphidophyceae) in Kagoshima Bay, Japan (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In April 1995, an unusual large-scale red tide of Heterosigma akashiwo (Hada) Hada occurred, causing massive fish kills in Kagoshima Bay, Japan. Four neurotoxic components, HaTx-i, HaTx-iia, HaTx-iib and HaTx-iii, which corresponded to brevetoxin components. PbTx-2. PbTx-9, PbTx-3 and oxidized PbTx-2, were inferred from analysis of Heterosigma red tide toxins on TLC and HPLC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1997.t01-1-00823.x
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  • 4
    Electronic Resource
    Electronic Resource
    Synthetic surfactants for protecting cultured fish against toxic phytoplankton (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 29 (1998), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Polyoxyethylene alkyl esters, which are surface-active agents, were chemically synthesized from fatty acids (C12-C18) on reaction with different moles of polyethylene oxides, and were tested for effectiveness against the toxic raphidophytes Chattonella marina (Subrahmanyan) Hara & Chihara and C. antiqua (Hada) Ono. The synthetic surfactants destroyed cultured cells from these two species. Although the synthetic surfactants also exhibited ichthyotoxicity, this was lowered by increasing the molarity of ethylene oxide (EO) in alkyl ester molecules. Young yellowtail, Seriola quinqueradiata Temminck & Schlegel, survived exposure to C. marina (5000-5500 cells mLT−1) or C. antiqua (3000-3500 cells mL−1) cultures with the addition of 4-5 p.p.m. oleyl ester EO 14, but died within an hour without the addition of this surfactant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1998.00239.x
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of tetrodotoxin by thin-layer chromatography/fast atom bombardment mass spectrometry
    Amsterdam : Elsevier
    Analytical Biochemistry 175 (1988), S. 258-262 
    Details
    ISSN: 0003-2697
    Keywords: TLCFAB mass spectrometry ; anhydrotetrodotoxin ; fast atom bombardment mass spectrometry ; tetrodonic acid ; tetrodotoxin ; thin-layer chromatography
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(88)90386-7
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of plant growth regulators on somatic embryogenesis in leaf cultures of Coffea canephora (1991)
    Springer
    Plant cell reports 10 (1991), S. 179-182 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of plant growth regulators on somatic embryogenesis were studied in leaf cultures of Coffea canephora. The maximum number of somatic embryos were obtained on media that contained only cytokinin as a plant growth regulator. All of the auxins tested (NAA, IBA, IAA and 2, 4-D) inhibited the formation of embryos. The optimal concentration of each cytokinin (2-iP, BA and kinetin) for somatic embryogenesis was 5 μM. Under optimal conditions, each explant formed more than 100 embryoids with little callus and few adventitious roots. Embryoids were formed only at the cut edges of the leaf discs. Cytokinins were absorbed only at the cut edges of leaf discs that were in contact with the medium, and were not transported to other parts of the explant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234290
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  • 7
    Electronic Resource
    Electronic Resource
    Tetrodotoxin-producing bacteria from the blue-ringed octopus Octopus maculosus (1989)
    Springer
    Marine biology 100 (1989), S. 327-332 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several live specimens of the blue-ringed octopus Octopus maculosus were collected from the Philippines in November 1985, and from Japan in February 1986, and the distribution of toxicity, along with toxin composition, in the posterior salivary gland and other soft parts were examined. Tetrodotoxin (TTX: 1400 mouse units g-1) was detected in the posterior salivary gland of a Japanese specimen, while not only the salivary gland but other soft parts were toxic in the Philippine specimens. The Philippine specimens contained TTX and anhydrotetrodotoxin, the Japanese specimen TTX, 4-epitetrodotoxin, and an unknown toxin. The posterior salivary gland, intestine and other parts were excised from the Philippine specimens and examined for bacterial flora. Twenty-two dominant strains were isolated and cultured in a 2xORI medium (Ocean Research Institute, Simidu and Tsukamoto 1985) at 20°C for 20 to 48 h. Cells were harvested by centrifugation, and disrupted by ultrasonication. The toxins were partially purified from the cell lyzate by ultrafiltration and Bio-Gel P-2 column-chromatography. Instrumental analyses disclosed that 16 of the 22 strains produced TTX and/or related substances. Six strains which clearly exhibited TTX productivity were identified as Alteromonas (2 strains), Bacillus (2), Pseudomonas (1) and Vibrio (1), based on biochemical and biological characteristics. Of these, one strain each of Bacillus and Pseudomonas produced TTX at a level detectable by the mouse assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391147
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  • 8
    Electronic Resource
    Electronic Resource
    Vibrio alginolyticus, a tetrodotoxin-producing bacterium, in the intestines of the fish Fugu vermicularis vermicularis (1987)
    Springer
    Marine biology 94 (1987), S. 625-630 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To clarify the mechanism of toxification in animals contaminated with tetrodotoxin, the intestinal contents of the puffer Fugu vermicularis vermicularis were examined for bacterial flora in 1985. Twenty-six out of 33 strains belonged to the genus Vibrio. These bacteria were classified into Groups I to VII, based on biological and biochemical characters. High performance liquid chromatography and gas chromatography-mass spectrometry, together with mouse bioassay for toxicity, clearly demonstrated that Group I produced tetrodotoxin and anhydrotetrodotoxin under cultivation with a medium composed of Phytone peptone (BBL) and NaCl. Some other groups also produced this toxin and/or related substances to some extent. Strains of Group I were all identified as Vibrio alginolyticus. Two strains among four produced a detectable amount of tetrodotoxin and/or anhydrotetrodotoxin, as measured by all instrumental analyses applied. Our findings suggest that some strains of V. alginolyticus are closely related to the toxification of the puffer, and probably of other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431409
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  • 9
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    Climate modelling study on mountain uplift and Asian monsoon evolution (2010)
    In:  Geological Society Special Publication 342: 293-301.
    Details
    Publication Date: 2010-08-16
    Description: Impacts of mountain uplift on the Asian monsoon and adjacent seas are investigated by climate model sensitivity studies. Two sets of general circulation model (GCM) experiments are performed. Using an atmosphere-ocean coupled GCM, a progressive mountain uplift experiment is performed. During boreal summer, monsoon precipitation is confined in the deep tropics around 10{degrees}N in the no-mountain case, but as mountains become higher, heavy rain areas move inland from the East Asian coast with stronger upward winds and increased rainfall over the southeastern Tibetan Plateau region. An increase of freshwater discharge from the Asian rivers results in a significant decrease of sea surface salinities over the Bay of Bengal, the South China, East China and Yellow Seas. A high-resolution atmospheric GCM experiment, which shows improvement in reproducing the present-day model climatology, gives more precise information on precipitation and the circulation changes caused by mountain uplift.
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  • 10
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    Tetrodotoxin-producing bacteria from the blue-ringed octopus Octopus maculosus (1989)
    Springer
    Details
    Publication Date: 1989-02-01
    Print ISSN: 0025-3162
    Electronic ISSN: 1432-1793
    Topics: Biology
    Published by Springer
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