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  • 2000-2004  (28)
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  • 1
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : We examined the ability of stationary phase Salmonella typhimurium to adhere and invade cultured HEp-2 cells after growth in broth supplemented with acetate, propionate, butyrate, or a mixture of the three short chain fatty acids (SCFA). At pH 6, all concentrations, except 25 and 50 mM butyrate, reduced cell-association of S. typhimurium when compared to controls, while at pH 7 only 100 mM concentrations of acetate and butyrate reduced cell-association significantly. Invasion percentages were greater for S. typhimurium grown at an initial pH of 6 with 25 mM acetate when compared to controls, SCFA mixture, and all other single SCFA concentration combinations. At pH 7, invasion was greater with either the SCFA mixture or 25 or 50 mM acetate than with control and all other single SCFA concentration combinations.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Short-chain fatty acids (SCFA), which are widely used as food preservatives and are also present in the gastrointestinal (GI) tract of animals at high concentrations, may play a role in the persistence of Salmonella typhimurium in the environment. To test the hypothesis, S. typhimurium was adapted to SCFA for 1 h and the % survivors against various stress conditions was determined. For adaptation, the SCFA mixtures at the concentrations found in small (SI) and large intestine (LI) were used. The % survivors against extreme acid (pH 3.0), high osmolarity (2.5 M NaCl), and reactive oxygen (20 mM H2O2) was greatly increased by exposure to SCFA LI, but to a much less extent by SCFA SI. The results suggest that encountering SCFA by S. typhimurium in the large intestine of the host food animal or food materials treated with them may increase the persistence of S. typhimurium in food animal pre-and postharvest production by enhancing overall stress resistance.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Lysine Maillard reaction products (LMRP) were generated by autoclaving different concentrations and ratios glucose and lysine for 3 h. Effects of LMRP on the Escherichia coli lysine auxotroph (lys−) were examined in model systems. The slope and y-axis intercept of the standard curve in the absence of LMRP were significantly (p 〈 0.05) greater than in the presence of LMRP. Our study shows that LMRP can decrease the growth extent of lys−. The E. coli available lysine assay may provide the food industry with a practical approach to rapid and accurate estimation of lysine for least cost formulation in foods.
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  • 4
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of different organic acid compounds on the survival of a dry inoculum of Salmonella typhimurium marker strain added to poultry feed were determined. Organic acids were added as 1% Zn or Na salts (w/w) to poultry feeds and stored at room temperature for 9 days. Reduction of S. typhimurium populations from 0 to day 9 was not substantially different between poultry layer ration nonamended feed (96.76%) and Zn acetate (99.72%) or Zn propionate (99.60%) amended feed. However, over 90% reduction of S. typhimurium populations occurred in Zn acetate and Zn propionate amended feed by day 3 while poultry layer ration populations reached 90% reduction by day 5. S. typhimurium populations after 9 days were nearly 40% more reduced in Na propionate amended feed than Na acetate amended feed. The results of this study indicated 1% Zn compounds may have potential to reduce survival of S. typhimurium in a poultry diet during the early stages of storage.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 12 (2004), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Estimating availability of methionine is relevant to feed formulation since diets can be supplemented with crystalline methionine to meet the minimum requirements of rapidly growing birds. Bacterial assays have been developed to measure the bioavailable levels of several essential amino acids in feeds, including methionine. The E. coli methionine auxotroph strain used in this study exhibited a linear extent of growth response to increasing concentrations of methionine added to the minimal test media, in the range of 0 to 4 μg/mL. In addition the growth rates of the E. coli auxotroph were significantly (P 〈 0.01) different when the methionine concentrations were varied (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 μg/mL) in minimal media. To assay feeds, feed grade methionine was added to poultry feed mixtures and samples were diluted with M9 media. Using this assay for estimating crystalline methionine added to feed, the extent of growth of the methionine auxotroph was correlated with the levels of crystalline methionine supplemented in the feed (R2= 0.9873). For all supplementation levels methionine recovery percentages ranged from 71 to 80% indicating that the bacterial assay response to crystalline methionine was relatively constant in the presence of the feed matrix. The overall results indicate that the rapid detection of crystalline methionine added to feeds is possible using this E. coli methionine auxotroph growth-based assay.
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  • 6
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Induced molting through feed withdrawal can change the microenvironment of crop and ceca sufficiently to allow them to be the sites of Salmonella colonization in the chicken intestine. This study compares the denaturing gradient gel electrophoresis (DGGE) profiles of microbial crop and cecal communities among molted hens fed similar zinc acetate or zinc propionate amended molt diets to hens either undergoing feed withdrawal or hens full fed and not molted. Dendrograms of DGGE amplicon patterns indicated over 85% similarity of cecal communities between zinc acetate fed hens and zinc propionate fed hens and over 60% similarity of crop communities between zinc acetate fed hens and zinc propionate fed hens. Rapid comparison of complex gastrointestinal microflora profiles in laying hens fed similar diets is possible using DGGE.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 11 (2003), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Microbiological assays for determination of bioavailable lysine appear to have many advantages. However, since the developed assay is based on bacterial growth and considerable optical density (OD) is required to detect distinguishable differences in extent of growth, it can be time consuming. The purpose of this study was to explore the fluorescence as an alternative method to measure bacterial growth instead of OD and examine the possibility to shorten the time required for the lysine assay. An assay based on SYTO 9 green fluorescent DNA binding dye (Live/Dead BacLight Protocol, Molecular Probes) was used to stain all bacteria in a population. Additional experiments were carried out to determine the ability of fluorescence based on SYTO 9 to overcome problems associated with high nonbacterial background that contributes to OD. From this study it appears that using fluorescence based on SYTO 9 green fluorescent staining, the E. coli lysine auxotroph growth assay can be completed in 9 h instead of 11 h and has the advantage of improved detection sensitivity. Problems associated with interference by high background nonbacterial OD can be partially resolved by fluorescence.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 10 (2002), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Methionine is one of the essential and first limiting amino acids in animal nutrition. In this study, an Escherichia coli methionine auxotroph bacterial strain that exhibits a linear growth response to methionine concentrations was transformed with a plasmid containing genes encoding ampicillin resistance and bioluminescence in order to develop a microbiological technique for methionine quantitation. Transformants were selected based on antibiotic resistance and plasmid containing candidates were confirmed by restriction enzyme digestion and gel electrophoresis. To confirm the bioluminescent phenotype, video imaging of the strain using long exposure photography yielded colonies exhibiting bioluminescence. The strain was also tested in the presence of ampicillin supplemented media with increasing methionine concentrations and growth response (measured as optical density, OD), growth rates and methionine affinities were compared before and after transformation. Although the transformed E. coli methionine auxotroph exhibited somewhat different growth kinetic responses than the nontransformed strain, the standard curves used for estimating methionine concentrations were not different. Based on the results in this study the transformed bioluminescent strain could be used as an OD-based assay if bioluminescence equipment and materials are not available.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 11 (2003), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract  The purpose of this research was to develop a microtiter-based assay procedure for assessment of virulence gene response of Salmonella Typhimurium to fresh homogenized vegetables. The microtiter procedure was adapted from a assay for β-galactosidase activity of a hilA fusion strain of S. Typhimurium with volumes of all reagents adjusted for microtiter plate wells. S. Typhimurium exposed to pH 7 saline yielded a higher level of hilA expression than responses after incubation in pH 4, 5 and 6 saline. Bacterial cells exposed to broccoli extracts yielded the higher levels of hilA expression while bell pepper and tomato yielded the overall lowest amounts of hilA expression. From this study, it appears that fresh produce extracts have different abilities to significantly alter virulence response in Salmonella. In conclusion, the hilA fusion stain assay can be successfully adapted to microtiter plates for testing Salmonella virulence response to vegetable extracts.
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  • 10
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The objective of these experiments was to determine whether alternative molting diets would minimize Salmonella enterica serovar Entertitidis (S. Enteritidis) colonization in molting hens. Hens were randomly assigned to four treatment groups of 12 hens either full-fed (nonmolt, NM), molted by feed withdrawal (molt, M), a low calcium (LC containing 800 mg calcium), or LC diet supplemented with 110 mg zinc/ kg of diet (LC-ZN) in two trials. All hens were challenged orally with 10 5SE on day 4 of experiment. Hen body weight loss was significantly (P 〈 0.05) increased and ovarian weight was significantly (P 〈 0.05) decreased in hens fed the LC or LC-ZN diets compared to NM. Cecal lactic acid concentrations were significantly (P 〈 0.05) increased in hens fed alternative molting diets. Feed withdrawal molted hens exhibited significantly (P 〈 0.05) more S. Enteritidis positive and S. Enteritidis crop, cecal, and organ colonization than NM, LC and LC-ZN hens. Alternative molt diets retain sufficient fermentative activity to limit S. Enteritidis colonization and therefore may have potential to avoid the risk of increasing S. Enteritidis colonization associated with feed withdrawal.
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