Publication Date:
2001-03-27
Description:
Neurotrophins such as brain-derived neurotrophic factor (BDNF) are thought to be transferred from post- to presynaptic neurons and to be involved in the formation and plasticity of neural circuits. However, direct evidence for a transneuronal transfer of BDNF and its relation to neuronal activity remains elusive. We simultaneously injected complementary DNAs of green fluorescent protein (GFP)-tagged BDNF and red fluorescence protein into the nucleus of single neurons and visualized expression, localization, and transport of BDNF in living neurons. Fluorescent puncta representing BDNF moved in axons in the anterograde direction, though some moved retrogradely, and transferred to postsynaptic neurons in an activity-dependent manner.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kohara, K -- Kitamura, A -- Morishima, M -- Tsumoto, T -- New York, N.Y. -- Science. 2001 Mar 23;291(5512):2419-23.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Neurophysiology, Biomedical Research Center, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871 Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11264540" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Newborn
;
Antibodies
;
*Axonal Transport
;
Axons/*metabolism
;
Brain-Derived Neurotrophic Factor/genetics/*metabolism
;
Cell Nucleus/metabolism
;
Cells, Cultured
;
DNA, Complementary
;
Dendrites/metabolism
;
Immunohistochemistry
;
Luminescent Proteins/metabolism
;
Mice
;
Mice, Inbred C57BL
;
Microscopy, Fluorescence
;
Microtubule-Associated Proteins/analysis/immunology
;
Neurites/metabolism
;
Neuronal Plasticity
;
Neurons/drug effects/*metabolism
;
Plasmids
;
Protein Transport
;
Recombinant Fusion Proteins/metabolism
;
Synapses/*metabolism
;
Tetrodotoxin/pharmacology
;
Visual Cortex/cytology
;
tau Proteins/analysis/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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