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  • 1
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    Infectious disease: Mobilizing Ebola survivors to curb the epidemic (2014)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2014-12-19
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Epstein, Joshua M -- Sauer, Lauren M -- Chelen, Julia -- Hatna, Erez -- Parker, Jon -- Rothman, Richard E -- Rubinson, Lewis -- England -- Nature. 2014 Dec 18;516(7531):323-5. doi: 10.1038/516323a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Advanced Modeling, and director for systems science of the Johns Hopkins Systems Institute, Johns Hopkins University, Baltimore, Maryland, USA. J.M.E. is also external professor at the Santa Fe Institute, Santa Fe, New Mexico, USA. ; Department of Emergency Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA. ; Johns Hopkins Center for Advanced Modeling, Baltimore, Maryland, USA. ; Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. ; R Adams Cowley Shock Trauma Center, University of Maryland School of Medicine, Baltimore, Maryland, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25519116" target="_blank"〉PubMed〈/a〉
    Keywords: Epidemics/*prevention & control ; Health Personnel/*standards ; Hemorrhagic Fever, Ebola/epidemiology/*prevention & control/*therapy ; Humans ; Immunity ; Risk ; Social Stigma ; *Survivors
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
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  • 2
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    Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples (2013)
    Oxford University Press
    Details
    Publication Date: 2013-10-08
    Description: Comprehensive profiling of nucleic acids in genetically heterogeneous samples is important for clinical and basic research applications. Universal digital high-resolution melt (U-dHRM) is a new approach to broad-based PCR diagnostics and profiling technologies that can overcome issues of poor sensitivity due to contaminating nucleic acids and poor specificity due to primer or probe hybridization inaccuracies for single nucleotide variations. The U-dHRM approach uses broad-based primers or ligated adapter sequences to universally amplify all nucleic acid molecules in a heterogeneous sample, which have been partitioned, as in digital PCR. Extensive assay optimization enables direct sequence identification by algorithm-based matching of melt curve shape and Tm to a database of known sequence-specific melt curves. We show that single-molecule detection and single nucleotide sensitivity is possible. The feasibility and utility of U-dHRM is demonstrated through detection of bacteria associated with polymicrobial blood infection and microRNAs (miRNAs) associated with host response to infection. U-dHRM using broad-based 16S rRNA gene primers demonstrates universal single cell detection of bacterial pathogens, even in the presence of larger amounts of contaminating bacteria; U-dHRM using universally adapted Lethal-7 miRNAs in a heterogeneous mixture showcases the single copy sensitivity and single nucleotide specificity of this approach.
    Keywords: Nucleic acid amplification
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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