ALBERT

Description: We have identified a novel regulatory erythroid kinase (REDK) that is homologous to a family of dual-specificity kinases. The yeast homolog of REDK negatively regulates cell division, suggesting a similar function for REDK, which is primarily localized in the nucleus. REDK is present in hematopoietic tissues, such as bone marrow and fetal liver, but the RNA is expressed at significant levels only in erythroid or erythropoietin (EPO)-responsive cells. Two novel forms of cDNA (long and short) for REDK have been isolated that appear to be alternative splice products and imply the presence of polypeptides with differing amino termini. The ratio of short-to-long forms of REDK increases dramatically in CD34+ cells cultured with EPO, suggesting differing regulation and function for each form. REDK is predominantly found in nuclear, rather than cytoplasmic, protein extracts, and immunoprecipitated REDK is active in phosphorylating histones H2b, H3, myelin basic protein, and other coimmunoprecipitated proteins. Antisense REDK oligonucleotides promote erythroid colony formation by human bone marrow cells, without affecting colony-forming unit (CFU)-GM, CFU-G, or CFU-GEMM numbers. Maximal numbers of CFU-E and burst-forming unit–erythroid were increased, and CFU-E displayed increased sensitivity to suboptimal EPO concentrations. The data indicate that REDK acts as a brake to retard erythropoiesis.

Description: Ocean Drilling Program Site 1002 in the Cariaco Basin was drilled in the final two days of Leg 165 with only a short transit remaining to the final port of San Juan, Puerto Rico. Because of severe time constraints, cores from only the first of the three long replicate holes (Hole 1002C) were opened at sea for visual description, and the shipboard sampling was restricted to the biostratigraphic examination of core catchers. The limited sampling and general scarcity of biostratigraphic datums within the late Quaternary interval covered by this greatly expanded hemipelagic sequence resulted in a very poorly defined age model for Site 1002 as reported in the Leg 165 Initial Reports volume of the Proceedings of the Ocean Drilling Program. Here, we present for the first time a new integrated stratigraphy for Site 1002 based on the standard of late Quaternary oxygen-isotope variations linked to a suite of refined biostratigraphic datums. These new data show that the sediment sequence recovered by Leg 165 in the Cariaco Basin is continuous and spans the time interval from 0 to ~580 ka, with a basal age roughly twice as old as initially suspected from the tentative shipboard identification of a single biostratigraphic datum. Lithologic subunits recognized at Site 1002 are here tied into this new stratigraphic framework, and temporal variations in major sediment components are reported. The biogenic carbonate, opal, and organic carbon contents of sediments in the Cariaco Basin tend to be high during interglacials, whereas the terrigenous contents of the sediments increase during glacials. Glacioeustatic variations in sea level are likely to exert a dominant control on these first-order variations in lithology, with glacial surface productivity and the nutrient content of waters in the Cariaco Basin affected by shoaling glacial sill depths, and glacial terrigenous inputs affected by narrowing of the inner shelf and increased proximity of direct riverine sources during sea-level lowstands.

Description: We used paleomagnetic results from Sites 998, 999, 1000, and 1001 to estimate the paleolatitude of the Caribbean region over the past 80 m.y. The data include remanence measurements of split-core sections (typically 1.5 m long) and discrete samples (6-12 cm**3 in volume) from volcanic and sedimentary rocks. From these, we computed 15 new paleolatitude estimates for Sites 999 and 1001 on the Caribbean plate and three new paleolatitude estimates for Site 998 on the Cayman Rise, currently on the southern North American plate. One estimate from Site 1001 is based on 230 measurements made along split-core sections of basalt after demagnetization of 20-25 mT. The other 17 estimates are based on principal component analysis of demagnetization data from 438 discrete paleomagnetic samples from sedimentary units. Where necessary, the 18 new paleolatitude estimates are corrected for a polarity ambiguity bias that occurs when averaging paleomagnetic data from drill cores that have shallow inclinations and are not azimuthally oriented. We also investigated the contribution of additional biases that may arise from a compaction-related inclination error, which could affect the sedimentary units, though not the basalt units. Several lines of evidence, including the lack of a correlation between porosity (or water content) and inclination, indicate that the inclination error is small, if present at all. The results from Sites 999 and 1001 indicate that the Caribbean plate was 5°-15° south of its current position at ~80 Ma, possibly placing it directly over the equator in the Late Cretaceous. Although the data do not preclude changes in the rate of northward motion over the past 80 m.y., they are consistent with a constant northward progression at a rate of 18 km/m.y. Given the uncertainties in the data, rates of northward motion could be as low as 8 km/m.y. or as high as 22 km/m.y. These results are compatible with several existing models for the evolution of the Caribbean plate, including those that have the Caribbean plate originating in the Pacific Ocean west of subduction zones active in the Central American region during the Cretaceous, and those that have the Caribbean plate originating within the Central American region, though more than 1000 km west of its current position relative to North and South America.

Description: Drilling in the Caribbean Sea during Ocean Drilling Program Leg 165 has recovered a large number of silicic tephra layers and led to the discovery of three major episodes of explosive volcanism that occurred during the last 55 m.y. on the margins of this evolving ocean basin. The earliest episode is marked by Paleocene to early Eocene explosive volcanism on the Cayman Rise, associated with activity of the Cayman arc, an island arc that was the westward extension of the Sierra Maestra volcanic arc in southern Cuba. Caribbean sediments also document a major mid- to late Eocene explosive volcanic episode that is attributed to ignimbrite-forming eruptions on the Chortis Block in Central America to the west. This event is contemporaneous with the first phase of activity of the Sierra Madre volcanic episode in Mexico, the largest ignimbrite province on Earth. In the Caribbean sediments, a Miocene episode of explosive volcanism is comparable to the Eocene event, and also attributed to sources in the Central American arc to the west. Radiometric 40Ar/39Ar dates have been obtained for biotites and sanidines from 27 tephra layers, providing absolute ages for the volcanic episodes and further constraining the geochronology of Caribbean sediments. Volcanic activity of the Cayman arc is attributed to the northward subduction of the leading edge of the oceanic plate that carried the Caribbean oceanic plateau. Although the factors generating the large episodes of Central American explosive volcanism are unclear, we propose that they are related to contemporary major readjustments of plate tectonic configuration in the Pacific.