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  • Articles  (16)
  • Springer  (16)
  • 1995-1999  (16)
  • 1990-1994
  • 1905-1909
  • 1995  (16)
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  • Articles  (16)
Years
  • 1995-1999  (16)
  • 1990-1994
  • 1905-1909
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  • 1
    ISSN: 1432-1157
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract The hypothesis that variations of the Amazon plume are forced primarily by wind is further explored through a series of simplified numerical model simulations. The wind's role in the change in plume structure and the nature of this change are investigated for two events: a shift in wind direction from westward to southeastward and a reduction in magnitude of the westward wind speed. Under winds with a southeastward component, the plume is confined to below 5°N; this simulation represents a rare but illustrative event showing how the balance of forces is quickly adjusted under changing winds. The freshest portions of the plume expand eastward, but are confined near the river mouth, as observed. The cross-shelf and alongshelf dynamic balances are similar in magnitude to those with westward wind stress, but the balance between the equatorial jet and buoyancy-driven cross-shelf flow is altered, controlling a new along-shelf position of the front. During wind-relaxation events, the plume widens near the mouth as a result of strong, eastward cross-shelf velocities associated with an equatorial Kelvin wave.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-904X
    Keywords: gelatin capsules ; moisture content ; near-infrared spectrophotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and computational fluid dynamics 7 (1995), S. 427-428 
    ISSN: 1432-2250
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and computational fluid dynamics 7 (1995), S. 425-425 
    ISSN: 1432-2250
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 1050-1055 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Two rice α-amylase isozymes, AmylA and Amy3D, were produced by secretion from genetically engineered strains of Saccharomyces cerevisiae. They have distinct differences in enzymatic characteristics that can be related to the physiology of the germinating rice seed. The rice isozymes were purified with immunoaffinity chromatography. The pH optima for amy3D (pH optimum 5.5) and Amy1A (pH optimum 4.2) correlate with the pH of the endosperm tissue at the times in rice seedling development when these isozymes are produced. Amy3D showed 10–14 times higher reactivity to oligosaccharides than Amy1A. Amy1A, on the other hand, showed higher reactivity to soluble starch and starch granules than Amy3D. These results suggest that the isozyme Amy3D, which is expressed at an early stage of germination, produces sugars from soluble starch during the early stage of seed germination and that the isozyme Amy1A works to initiate hydrolysis of the starch granules.
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  • 6
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Genes responsible for genetic diseases with increased sensitivity to DNA-damaging agents can be identified using complementation cloning. This strategy is based on in vitro complementation of the cellular sensitivity by gene transfer. Ataxia-telangiectasia (A-T) is a multisystem autosomal recessive disorder involving cellular sensitivity to ionizing radiation and radiomimetic drugs. A-T is genetically heterogeneous, with four complementation groups. We attempted to identify cDNA clones that modify the radiomimetic sensitivity of A-T cells assigned to complementation group [A-T(A)]. The cells were transfected with human cDNA libraries cloned in episomal vectors, and various protocols of radiomimetic selection were applied. Thirteen cDNAs rescued from survivor cells were found to confer various degrees of radiomimetic resistance to A-T(A) cells upon repeated introduction, and one of them also partially influenced another feature of the A-T phenotype, radioresistant DNA synthesis. None of the clones mapped to the A-T locus on chromosome 11q22-23. Nine of the clones were derived from known genes, some of which are involved in cellular stress responses. We concluded that a number of different genes, not necessarily associated with A-T, can influence the response of A-T cells to radiomimetic drugs, and hence the complementation cloning approach may be less applicable to A-T than to other diseases involving abnormal processing of DNA damage.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 126 (1995), S. 839-850 
    ISSN: 1434-4475
    Keywords: Electrode glasses ; Principal Component Analysis ; Cluster analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Cluster Analysis has been shown to be partially suitable for the classification of glasses. The expansion of the clusters on theSeger's diagram allows an investigation of the influence of the different glass components. Variation of the SiO2 content results in a smaller change of properties than variation of the Na2O/RO relationship. Principal Component Analysis is convenient for showing correlations between the composition of the examined glasses, the glass structure parameters, and the different electrode properties. Properties of electrodes are mostly influenced by the content of Na2O and by the kind of the divalent cations. In contrast thereto structure parameters become basically determined by the content of SiO2 and RO. The field strength of the divalent cations plays the decisive roll again.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 173 (1995), S. 329-335 
    ISSN: 1573-5036
    Keywords: calcium sulfite ; flue gas desulfurization residue ; oxidation ; sulfur dioxide ; toxicity ; Triticum aestivum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract CaSO3 is a by-product formed by several of the processes used for scrubbing SO2 from flue gas produced by coal-burning power generators. Using CaSO3 to improve the calcium status of acid soils would be a beneficial alternative to disposal in landfills. CaSO3 has biocidal properties and is used as a disinfectant and food and drink preservative. It is important to evaluate under what conditions application to soils would not harm plant growth. Laboratory experiments confirmed that two transformations of CaSO3 occurred in soil systems: (1) decomposition to produce SO2 gas, and (2) oxidation to calcium sulfate. Conversion to SO2 occurred in solution and soil at low pH, and acid soils treated with CaSO3 were initially toxic to seedling root growth. The degree of toxicity was time-dependent, with reduction in toxicity occurring as CaSO3 oxidized to calcium sulfate. Soil reaction also influenced toxicity, and at soil pH levels above 6, little seedling toxicity was evident.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 145 (1995), S. 131-139 
    ISSN: 1573-4919
    Keywords: glycogen phosphorylase ; alloxan-diabetes ; cardiomyocytes ; cGMP ; phosphodiesterase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The focus of this study was to identify the molecular basis for the hypersensitive response of glycogen phosphorylase activation to epinephrine stimulation in alloxan diabetic-derived cardiomyocytes. Cyclic AMP levels were found not to be significantly different between normal and diabetic-derived cells while cGMP concentrations were found consistently to be significantly lower in diabetic-derived cells than in normal cells. Treatment with cyclic GMP analogues did not affect phosphorylase activation by epinephrine in normal cardiomyocytes whereas, IBMX, a nonselective phosphodiesterase inhibitor, had a significant effect on basal and agonist-stimulated phosphorylase activity in both normal and diabetic-derived cardiomyocytes. Differences in the time course for the rate of decay of phosphorylasea from agonist-stimulated to basal levels were observed between normal and diabetic cells. After 3 h in primary culture, phosphorylasea activity returned to basal levels more quickly in normal than in diabetic-derived cells while after 24 h in culture, the time for phosphorylasea decay was not significantly different between normal and diabetic myocytes and was longer than the 3 h response. After 3 h in primary culture, no significant difference in phosphorylase kinase activity was observed between normal and diabetic-derived cells exposed to epinephrine whereas, after 24 h in culture, phosphorylase kinase activity was significantly decreased in diabetic cells under basal and agonist-stimulated conditions. These data collectively suggest that the hypersensitive response of glycogen phosphorylase to epinephrine stimulation in diabetic-derived cardiomyocytes is not due to a defect present at the level of phosphorylase kinase but may, in part, result from an alteration in cardiac phosphodiesterase activity resulting from diminished intracellular cyclic GMP concentrations.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The protein journal 14 (1995), S. 559-566 
    ISSN: 1573-4943
    Keywords: Bovine somatotropin ; methemerythrin ; cytochrome b-562 ; cytochrome c′ ; CHARMM ; GROMOS ; UHBD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Molecular dynamics simulations and energy analysis have been carried out to study the structural mobility and stability of the four α-helix bundle motifs. The simulation results as well as the X-ray data show that the atomic RMS fluctuation is larger at the loop region for four representative proteins investigated: methemerythrin, cytochrome b-562, cytochrome c′, and bovine somatotropin. The loop-loop, helix-helix, and loop-helix interactions are computed for the unfolded and folded proteins. In the folded and solvated protein structures the loop-helix interaction is stronger than the helix-helix interaction, especially in the electrostatic component. But the stabilization energies of both the loop-helix and the helix-helix interactions relative to the those of an unfolded structure are of the same order of magnitude. The stabilization due to protein-solvent interaction is greater in the helix region than in the loop region. The percentage of hydrophilic solvent accessible area for the four proteins studied was calculated with the method of Eisenberg and McLachlan. The percentage of the hydrophilic area is greater in the loops than in the helices. A Poisson-Boltzmann calculation shows that the potential from the loops acting on a helix is generally more negative than that from other helices.
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