Publication Date:
2012-01-01
Description:
Static broth culture favorsSalmonella entericasubsp.entericaserovar Typhimurium to produce type 1 fimbriae, while solid agar inhibits its expression. A transposon inserted instbC, which would encode an usher for Stb fimbriae of a non-flagellarSalmonella entericasubsp.entericaserovar Typhimurium LB5010 strain, conferred it to agglutinate yeast cells on both cultures. RT-PCR revealed that the expression of the fimbrial subunit genefimA, andfimZ, a regulatory gene offimA, were both increased in thestbCmutant when grown on LB agar;fimW, a repressor gene offimA, exhibited lower expression. Flagella were observed in thestbCmutant and this phenotype was correlated with the motile phenotype. Microarray data and RT-PCR indicated that the expression of three genes,motA, motB, andcheM, was enhanced in thestbCmutant. ThestbCmutant was resistant to several antibiotics, consistent with the finding that expression ofyhcQandramAwas enhanced. A complementation test revealed that transforming a recombinant plasmid possessing thestbCrestored the mannose-sensitive agglutination phenotype to thestbCmutant much as that in the parentalSalmonella entericasubsp.entericaserovar Typhimurium LB5010 strain, indicating the possibility of an interplay of different fimbrial systems in coordinating their expression.
Print ISSN:
2356-6140
Electronic ISSN:
1537-744X
Topics:
Natural Sciences in General
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