ISSN:
0935-6304
Keywords:
High-performance thin-layer chromatography, HPTLC
;
Reversed-phase
;
Octadecyl-bonded silica gel
;
Bile acid methyl esters
;
Methyl 5β -cholanates
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A comparative study is reported on separation of series of mono-, di-, and trisubstituted methyl 5β-cholanates, which differ only in the position and stereochemistry of hydroxyl or keto groups at position and stereochemistry of hydroxyl or keto groups at positions C-3, C-7, and/or C-12, by reversed-phase [with chemically-bonded (C-18) silica gel] and normal-phase (silica gel) high-performance thin-layer chromatography (HPTLC). Methnol (or acetonitrile)/water systems were employed as mobile phase. Reversed-phase HPTLC found to be particulary effective for separation of the stereoisomers of di- and trisubstituted compounds whereas the less polar monosubstituted isomers are well resolved in normal-phase HPTLC.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240061106
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