Publication Date:
2012-04-12
Description:
Polycomb group (PcG) proteins control development and cell proliferation through chromatin-mediated transcriptional repression. We describe a transcription-independent function for PcG protein Posterior sex combs (PSC) in regulating the destruction of cyclin B (CYC-B). A substantial portion of PSC was found outside canonical PcG complexes, instead associated with CYC-B and the anaphase-promoting complex (APC). Cell-based experiments and reconstituted reactions established that PSC and Lemming (LMG, also called APC11) associate and ubiquitylate CYC-B cooperatively, marking it for proteosomal degradation. Thus, PSC appears to mediate both developmental gene silencing and posttranslational control of mitosis. Direct regulation of cell cycle progression might be a crucial part of the PcG system's function in development and cancer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mohd-Sarip, Adone -- Lagarou, Anna -- Doyen, Cecile M -- van der Knaap, Jan A -- Aslan, Ulku -- Bezstarosti, Karel -- Yassin, Yasmin -- Brock, Hugh W -- Demmers, Jeroen A A -- Verrijzer, C Peter -- New York, N.Y. -- Science. 2012 May 11;336(6082):744-7. doi: 10.1126/science.1215927. Epub 2012 Apr 5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Centre for Biomedical Genetics, Erasmus University Medical Centre, Post Office Box 1738, 3000 DR, Rotterdam, Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22491092" target="_blank"〉PubMed〈/a〉
Keywords:
Anaphase-Promoting Complex-Cyclosome
;
Animals
;
Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome
;
Carrier Proteins/metabolism
;
*Cell Cycle Checkpoints
;
Cell Line
;
Compound Eye, Arthropod/growth & development/metabolism
;
Cyclin B/*metabolism
;
DNA-Binding Proteins/chemistry/genetics/*metabolism
;
Drosophila Proteins/chemistry/genetics/*metabolism
;
Drosophila melanogaster/cytology/embryology/metabolism
;
G2 Phase Cell Cycle Checkpoints
;
Gene Silencing
;
Imaginal Discs/metabolism
;
*Mitosis
;
Phenotype
;
Polycomb Repressive Complex 1
;
Proteasome Endopeptidase Complex/metabolism
;
Protein Binding
;
Protein Interaction Domains and Motifs
;
RNA Interference
;
Transcription, Genetic
;
Ubiquitin-Protein Ligase Complexes/metabolism
;
Ubiquitination
;
Wings, Animal/growth & development
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
