ISSN:
1573-5079
Keywords:
cytochrome bc 1 complex
;
Rieske iron-sulfur protein
;
photosynthetic bacteria
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cytochrome bc 1 complexes have been isolated from wild type Rhodopseudomonas viridis and Rhodospirillum rubrum and purified by affinity chromatography on cytochrome c-Sepharose 4B. Both complexes are largely free of bacteriochlorophyll and carotenoids and contain cytochromes b and c 1 in a 2:1 molar ratio. For the Rps. viridis complex, evidence has been obtained for two spectrally distinct b-cytochromes. The R. rubrum complex contains a Rieske iron-sulfur protein (present in approximately 1:1 molar ratio to cytochrome c 1) and catalyzes an antimycin A- and myxothiazol-sensitive electron transfer from duroquinol to equine cytochrome c or R. rubrum cytochrome c 2. Although an attempt to prepare a cytochrome bc 1 complex from the gliding green bacterium Chloroflexus aurantiacus was not successful, membranes isolated from phototrophically grown Cfl. aurantiacus were shown to contain a Rieske iron-sulfur protein and protoheme (the prosthetic group of b-type cytochromes).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00029743
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