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  • 1
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    Field Comparison of Dinitrogen Fixation Determined with Nitrogen‐15‐Depleted and Nitrogen‐15‐Enriched Ammonium Sulfate in Selected Inbred Backcross Lines of Common Bean (1988)
    Wiley
    Details
    Publication Date: 1988-09-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 2
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    Indirect measures of N2 fixation in common bean (Phaseolus vulgaris L.) under field conditions: The role of lateral root nodules (1989)
    Springer
    Details
    Publication Date: 1989-01-01
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 3
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    Distribution of nitrogen in common bean (Phaseolus vulgaris L.) genotypes selected for differences in nitrogen fixation ability (1991)
    Springer
    Details
    Publication Date: 1991-12-01
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 4
    Electronic Resource
    Electronic Resource
    Potential role for salicylic acid in induced resistance of asparagus roots to Fusarium oxysporum f.sp. asparagi (2005)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 54 (2005), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Pre-inoculation of asparagus (Asparagus officinalis) roots with selected nonpathogenic isolates of Fusarium oxysporum (npFo) has previously been shown to induce systemic resistance against infection by F. oxysporum f.sp. asparagi (Foa) through activation of plant-defence mechanisms. To elucidate the putative npFo-mediated defence pathways, the effect of salicylic acid (SA) was examined in a split-root system of asparagus where one half of the seedling root system was drenched with SA and the activation of defence responses was measured subsequently on the remaining roots. SA-treated plants exhibited enhanced systemic resistance, with a significant reduction in disease severity of the roots inoculated with Foa, compared with untreated plants. SA activated peroxidase and phenylalanine ammonia-lyase, as well as lignification, upon Foa attack, in a manner similar to that observed with npFo pretreatment. In addition, application of diphenyleneiodonium, an SA biosynthesis inhibitor, led to failure of npFo to induce lignin deposition and systemic resistance. Treatment of fungal spores with SA did not affect germination and growth of either npFo or Foa in in vitro antifungal assays. Production of SA at the site of npFo infection may be involved in the induction of Foa resistance in asparagus roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.2005.01163.x
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  • 5
    Electronic Resource
    Electronic Resource
    Induction of systemic disease resistance and pathogen defence responses in Asparagus officinalis inoculated with nonpathogenic strains of Fusarium oxysporum (2002)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 51 (2002), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The ability of nonpathogenic isolates of Fusarium oxysporum (npFo) to induce systemic resistance and defence responses against subsequent challenge with a pathogenic strain of F. oxysporum f. sp. asparagi (Foa) was examined in Asparagus officinalis. In a split-root experiment, roots inoculated with npFo exhibited a hypersensitive response and those subsequently inoculated with Foa displayed resistance. Induction of systemic resistance in npFo-treated plants led to significantly fewer necrotic lesions (P = 0·05) and reduced Foa disease severity compared with plants not treated with npFo. In hyphal-sandwich root inoculation experiments, activities of peroxidase and phenylalanine ammonia-lyase and lignin content were higher in npFo-treated plants and increased more rapidly than in npFo-untreated plants after Foa inoculation. Antifungal activity (inhibition of fungal spore germination and germ-tube growth) from exudates of roots inoculated with Foa were observed for npFo-treated plants but not for npFo-untreated plants. Thus, isolates of npFo may function as inducers of systemic acquired resistance (SAR) and defence responses against Foa invasion in A. officinalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2002.00682.x
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  • 6
    Electronic Resource
    Electronic Resource
    High frequency production of haploid embryos in asparagus anther culture (1991)
    Springer
    Plant cell reports 10 (1991), S. 574-578 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method for obtaining a high frequency of haploid asparagus embryos through anther culture was developed. Flowers collected from plants in the field in July, August and September 1990, for the genotype G203, were stored at 5°C for 24 h. Anthers were placed on Murashige and Skoog medium (MS) containing 500 mg l −1 casein hydrolysate, 800 mg l−1 glutamine, 2 mg l −1 NAA, 1 mg l −1 BA and 5 % sucrose at 32 °C in the dark for three to four weeks to induce calli. Calli were then grown at 25 °C with a 16 h photoperiod for three to four weeks. Developing embryos and calli were transferred to embryo maturation medium, MS containing 6% sucrose, 0.1 mg l −1 NAA, 0.1 mg l −1 kinetin and 0.65 mg l −1 ancymidol, for four weeks. More than 50% of the recovered mature embryos germinated on MS containing l mg l −1 GA3. Anthers with microspores at the late-uninucleate stage had the highest frequency of total and embryogenic calli formation, 40% and 15%, respectively. Each embryogenic callus usually produced 10–15 embryos. Aproximately 75 plants per 100 anthers cultured were recovered: 76% haploid, 22% diploid and 2% triploid. High temperature was critical for the induction of embryogenic callus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232514
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  • 7
    Electronic Resource
    Electronic Resource
    Development of haploid asparagus embryos from liquid cultures of anther-derived calli is enhanced by ancymidol (1993)
    Springer
    Plant cell reports 12 (1993), S. 281-285 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of ancymidol concentration on the development of haploid asparagus embryos was determined. Liquid cultures from anther-derived calli were grown for three weeks in MS medium plus 1.0 mg l−1 2,4-D, 0.1 mg l−1 NAA, 0.2 mg l−1 kinetin, 800 mg l−1 glutamine, 500 mg l−1 casein hydrolysate, 2% sucrose and 0.0–1.0 mg l−1 ancymidol. Cell clumps (224–500 μm) were plated on solid embryo maturation medium (MS medium plus 3% sucrose, 0.1 mg l−1 NAA, 0.5 mg l−1 kinetin and 0.0–1.0 mg l−1 ancymidol) and grown for eight weeks. Ancymidol enhanced embryo maturation and germination and was more critical in the solid than liquid medium. Total embryo number did not vary among most treatments. The best response was observed when ancymidol concentrations were 0.1 and 0.5 mg l−1 in the liquid and solid media, respectively; two-thirds of the embryos produced were bipolar and 35% of bipolar embryos germinated. Seven to 82% of plants recovered from different ancymidol treatments were haploid; the others were diploid, triploid or chimeric for ploidy level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237136
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  • 8
    Electronic Resource
    Electronic Resource
    Improved callus formation and plant regeneration for shed microspore culture in asparagus (Asparagus officinalis L.) (1999)
    Springer
    Plant cell reports 18 (1999), S. 954-958 
    Details
    ISSN: 1432-203X
    Keywords: Key words Asparagus ; Shed microspore culture ; Androgenesis ; Cell division ; Callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To establish an efficient asparagus microspore culture system, experiments were conducted to investigate the effects of medium components, period of cold pretreatment for flower buds, and period of anther co-culture on culture response. All factors affected the frequency of asparagus microspore division and the yields of microspore-derived calli. The best results were obtained by pretreating genotype G459 flower buds at 4  °C for 7–9 days, co-culturing anthers with shed microspores for 14 days, and including 6% sucrose, 2 mg l–1α-naphthaleneacetic acid and 1 mg l–1 N6-benzylaminopurine in the culture medium. After 4 days of culture, most shed microspores contained starch-like bodies and died. The 2% of shed microspores lacking these structures divided to produce microcalli. For the best treatments in the different experiments, about 140 calli per 100 anthers were recovered. Cultured on four different regeneration media, 19.6–21% and 3.9–8.0% of microspore-derived calli produced shoots and embryos, respectively, and ultimately plantlets, among which 49% were haploid, 34% diploid, 4% triploid and 11% tetraploid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050690
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  • 9
    Electronic Resource
    Electronic Resource
    Interactions of ancymidol with sucrose and α-naphthaleneacetic acid in promoting asparagus (Asparagus officinalis L.) somatic embryogenesis (1997)
    Springer
    Plant cell reports 16 (1997), S. 879-883 
    Details
    ISSN: 1432-203X
    Keywords: Key words Ancymidol ; Sucrose ; α-Naphthaleneacetic acid ; Asparagus officinalis ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interactions of varying ancymidol concentrations with those of α-naphthaleneacetic acid (NAA) or sucrose in embryo induction medium were related to the production and development of asparagus (Asparagus officinalis L.) somatic embryos, and to the ability of these embryos to germinate. A significant sucrose×ancymidol interaction was observed only for the production of bipolar embryos; 4% sucrose with 0.75 mg l–1 ancymidol gave the best result, 78 g–1 callus. The frequency of globular embryos decreased as sucrose or ancymidol concentrations increased. Sucrose concentration affected embryo germination; 3% and 4% sucrose were optimal with approximately 60% and 40% of bipolar and globular embryos germinating, respectively. Significant ancymidol×NAA interactions were observed for the production of bipolar and globular embryos and their germination. Varying ancymidol concentrations affected embryo production and germination in combination with 0.1 mg l–1 NAA, but not with 1.0 mg l–1 NAA. The treatment combination of 0.1 mg l–1 NAA with 0.75 mg l–1 ancymidol produced the most bipolar embryos, 64 g–1 callus, and the greatest percentages of bipolar and globular embryos germinated, 63% and 42%, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050338
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  • 10
    Electronic Resource
    Electronic Resource
    Chromosome doubling via tuber disc culture in dihaploid potato as determined by confocal microscopy (1997)
    Springer
    Plant cell reports 16 (1997), S. 329-333 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The potential of tuber disc culture for chromosome doubling was investigated in somaclonal populations of four dihaploid genotypes and one tetraploid cultivar of potato (Solanum tuberosum). Laser scanning confocal microscopy (LSCM) was used for rapid determination of the ploidy level based on the number of chloroplasts in stomatal guard cells of leaves. Factorial analysis of chloroplast number in 58 clones and two leaf types showed that somaclones were clearly divided in two groups. Clones with 5–7 chloroplasts per cell as observed in tuber derived diploid controls were classified as 2X (not doubled), while those with 9–14 chloroplasts resembled the tuber derived tetraploid controls and were considered 4X (doubled). A high frequency of spontaneous chromosome doubling, 42% – 50%, was detected in 3 dihaploid genotypes, whereas no doubling was observed in one of the dihaploids as well as the tetraploid cultivar Yukon Gold. Effects of leaf type on chloroplast number was also significant. The middle leaf showed significantly higher chloroplast number than the younger leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01088291
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