ISSN:
1572-8781
Keywords:
patterning
;
recording
;
hippocampal
;
photoresist
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
Abstract Recent advances in cell biology and surface patterning make possible the construction of in vitro neural networks for long-term, multichannel recording studies. Towards this goal, we have demonstrated the recording of spontaneous electrical activity from rat embryonic hippocampal neurons confined to parallel lines which overlay the microelectrode array. The neurons adhered to adsorbed poly-D-lysine patterns and remained alive on the pattern for up to one month. Recordable, extracellular electrical activity began as early as 6 days in vitro and continued for the duration of the culture. Average amplitude of detected action potentials ranged between 70 μ V to 150 μ V measured from baseline to peak, consistent with results from unpatterned culture technologies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009946920296
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