ISSN:
0006-3592
Keywords:
subtilisin
;
chymotrypsin
;
substrate specificity
;
organic solvents
;
lyophilized enzymes
;
stereoselectivity
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme preparations, predicts that the kcat/KM ratio for two substrates should be proportional to their Raoult's law activity coefficients. This approach has been validated for two enzymes, subtilisin Carlsberg and α-chymotrypsin, catalyzing the propanolysis of unnatural (in addition to natural) ester substrates in a variety of anhydrous solvents. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 340-344, 1997.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
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