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  • 1
    ISSN: 1617-4623
    Keywords: Protoplast fusion ; Nitrate reductase deficiency ; Kanamycin ; Nicotiana tabacum ; Nicotiana sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The combination in the nuclear genome of a dominant resistance marker (to select against unfused wild-type cells) and a recessive deficiency marker (to select against unfused mutant cells) in a cell line should provide a system for selecting fusion hybrids between the mutant line and any wild-type line. To test this idea, we fused protoplasts from a non-morphogenic cell line of Nicotiana tabacum which was kanamycin resistant (by transformation) and deficient in nitrate reductase (NR-K+) with protoplasts from N. tabacum cv. Petit Havana clone SR1, which provided resistance against streptomycin as an additional selectable marker (NR+K-SR+). Putative hybrids were selected using a culture medium containing no available reduced nitrogen source and 50 mg/l kanamycin sulphate. After regeneration into plants, the hybrid character was demonstrated from: (i) the morphological variation of the regenerants; (ii) the chromosome number; (iii) the ability to grow on medium without a reduced nitrogen source and containing kanamycin sulphate at 50 mg/l; (iv) the presence of nitrate reductase activity; (v) the presence of the gene coding for neomycin phosphotransferase, which provides resistance to kanamycin sulphate; (vi) callus formation from leaves on medium containing 1 g/l streptomycin or 50 mg/l kanamycin sulphate; (vii) F1 plants containing nitrate reductase and the gene for neomycin phosphotransferase. Fusions between the mutant cell line (NR-K+) and three wild-type tobacco species and subsequent cultivation on medium containing no available nitrogen source but 50 mg/l kanamycin sulphate resulted in callus formation with all combinations, while hybrid plants were only regenerated when N. sylvestris was the fusion partner.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 1980-01-01
    Description: Pollen analysis as well as 18O/16O results on lake marl show that the Younger Dryas climatic period, between about 11,000 and 10,300 bp, was the last vigorous cold phase of the Würm Glacial. Detailed 14C analyses from a peat bog near Wachseldorn (Switzerland) point to a 14C anomaly in this period. Further indication of a 14C anomaly is given by the observation that, during the Younger Dryas period, the sedimentation rates in several lakes apparently were higher than in adjacent periods; an explanation might be that the 14C time scale was compressed between 11,000 and 10,000 bp, ie, the atmospheric 14C/C ratio varied. If real, this suggested 14C variation would probably be connected to the climatic events during this transition period from Later Glacial to Postglacial.
    Print ISSN: 0033-8222
    Electronic ISSN: 1945-5755
    Topics: Archaeology , Energy, Environment Protection, Nuclear Power Engineering , Geosciences
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  • 3
    Publication Date: 1995-01-01
    Description: Auf der südwestlich des Städtchens Huttwil (Schweizerisches Mittelland) gelegenen Terrasse von Schweinbrunnen wurde mit einer Rotationskernbohrung ein 11,60 m langes Profil mit Schieferkohleflözen gewonnen und pollenanalytisch untersucht. Dabei wurde ein nahezu vollständiges Interglazial erläßt, das aufgrund palynostratigraphischer Befunde dem letzten Interglazial (Riss/Würm) zuzuordnen ist. Zwei fragmentarische Sequenzen aus Sedimenten im Hangenden der Schieferkohlen zeigen kurze Ausschnitte aus Pinus/Picea-Interstadialen. Die Ablagerung der Schieferkohlen setzte kurz, nach dem Beginn der Abies-Phase ein und kam am Ende des Interglazials zum Stillstand. Die frühen Phasen des Interglazials waren durch starke Erosions- und Akkumulationsvorgänge gekennzeichnet. Größere Abtragungsvorgänge fanden ebenfalls während der Abies-Phase und am Übergang zur nachfolgenden Kaltzeit (Würm) statt.
    Print ISSN: 0424-7116
    Electronic ISSN: 2199-9090
    Topics: Geosciences , History
    Published by Copernicus on behalf of Deutsche Quartärvereinigung.
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  • 4
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    PANGAEA
    In:  European Pollen Database (EPD)
    Publication Date: 2023-02-07
    Keywords: Col Luitel, France; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Lithology/composition/facies; LUITEL; profil3
    Type: Dataset
    Format: text/tab-separated-values, 24 data points
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  • 5
    Publication Date: 2023-02-07
    Keywords: CREUX; Creux de Croue; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Lithology/composition/facies; Switzerland
    Type: Dataset
    Format: text/tab-separated-values, 3 data points
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  • 6
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    Unknown
    PANGAEA
    In:  European Pollen Database (EPD)
    Publication Date: 2023-02-08
    Keywords: CHIRENS1; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Lithology/composition/facies; TourbièredeChirens; Tourbière de Chirens, France
    Type: Dataset
    Format: text/tab-separated-values, 39 data points
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  • 7
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    Unknown
    PANGAEA
    In:  European Pollen Database (EPD)
    Publication Date: 2023-02-23
    Keywords: Age, dated; Age, dated, error to older; Age, dated, error to younger; Age, radiocarbon; Col Luitel, France; DEPTH, sediment/rock; LUITEL; profil3; Sample, optional label/labor no
    Type: Dataset
    Format: text/tab-separated-values, 12 data points
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  • 8
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    Unknown
    PANGAEA
    In:  European Pollen Database (EPD)
    Publication Date: 2023-11-01
    Keywords: Allium; Alnus glutinosa/A. incana; Artemisia; Asteroideae; Betula; Botrychium; Calluna; Caryophyllaceae; Centaurea jacea/C. scabiosa; Chenopodiaceae; CHIRENS1; Cichorioideae; Cirsium; Corylus; Cruciferae; Cyperaceae; DEPTH, sediment/rock; Dipsacaceae; Dryopteris; Dryopteris thelypteris; Ephedra distachya-type; Ephedra fragilis-type; Epilobium; Fabaceae; Filipendula cf. ulmaria; Geranium; Gramineae; Gypsophila repens; Hedera; Helianthemum alpestre; Helianthemum nummularium; Heracleum; Hippophae; Juniperus; Labiatae; Ligusticum; Liliaceae; Linum; Lycopodium; Nymphaeaceae; Oxyria-type; Parnassia; Pimpinella; Pinus; Plantago alpina; Plantago lanceolata; Plantago montana; Polemonium; Polygonum bistorta; Populus; Potamogeton; Quercus; Ranunculaceae; Rosaceae; Rubiaceae; Rumex-type; Salix; Sanguisorba minor; Sanguisorba officinalis; Saxifraga oppositifolia; Scleranthus; Selaginella; Sparganium; Sphagnum; Thalictrum; Tilia; TourbièredeChirens; Tourbière de Chirens, France; Typha; Ulmus; Umbelliferae/Apiaceae; Urtica; Valeriana dioica; Varia; Viburnum
    Type: Dataset
    Format: text/tab-separated-values, 7242 data points
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  • 9
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    Unknown
    PANGAEA
    Publication Date: 2023-11-01
    Keywords: Abies; Acer; Alnus; Anthemideae; Apiaceae; Artemisia; Betula; Botrychium; Brassicaceae; Calluna; Campanulaceae; Cannabis/Humulus; Carduus-type; Carpinus; Caryophyllaceae; Castanea; Centaurea cyanus; Centaurea jacea; Cerealia; Chenopodiaceae; Cichorioideae; Cornus mas; Cornus sanguinea; Corylus; Counting, palynology; Crataegus; CREUX; Creux de Croue; Cyperaceae; DEPTH, sediment/rock; Dipsacaceae; Drosera; Equisetum; Ericaceae; Euphorbiaceae; Eyepiece field of view; Fagus; Filipendula; Frangula; Fraxinus; Gentianaceae; Hedera; Helianthemum; Hypericum; Indeterminable: unknown; Iris; Juglans; Juniperus; Lamiaceae; Liliaceae; Lonicera; Lotus-type; Malvaceae; Mercurialis; Monolete spore(s); Number of counted lines; Nuphar; Oenotheraceae; Ononis-type; Papaver; Picea; Pinus; Plantago lanceolata; Plantago major/media; Poaceae; Pollen, total; Polygonum bistorta/P. viviparum; Polypodium; Populus; Potamogeton; Potentilla; Primulaceae; Prunus; Quercus; Ranunculaceae; Rhamnus catharticus; Rosaceae-type; Rubiaceae; Rumex; Salix; Sambucus; Sample mass; Sample volume; Sanguisorba minor; Sanguisorba officinalis; Saxifragaceae; Scrophulariaceae; Sorbus; Spores, trilete; Switzerland; Thalictrum; Tilia; Trifolium-type; Typha angustifolia; Typha latifolia; Ulmus; Urticaceae; Valerianaceae; Viburnum; Vicia-type
    Type: Dataset
    Format: text/tab-separated-values, 8640 data points
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  • 10
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    Unknown
    PANGAEA
    In:  European Pollen Database (EPD)
    Publication Date: 2023-11-01
    Keywords: Abies; Acer; Alnus; Apiaceae; Artemisia; Asteroideae; Betula; Botrychium; Brassicaceae; Calluna; Campanulaceae; Carpinus; Caryophyllaceae; Centaurea; Cerealia; Chenopodiaceae; Cichorioideae; Col Luitel, France; Corylus; Cyperaceae; DEPTH, sediment/rock; Dipsacaceae; Epilobium; Equisetum; Ericaceae; Fagus; Filipendula; Fraxinus; Geranium; Hedera; Helianthemum; Hippophae; Ilex; Juglans; Juniperus; Lamiaceae; Larix; Liliaceae; LUITEL; Lycopodium; Menyanthes; Monolete spore(s); Picea; Pinus; Plantago lanceolata; Poaceae; Polypodium; Populus; Potamogeton; profil3; Pteridium; Quercus robur-type; Ranunculaceae; Rosaceae; Rubiaceae; Rumex; Salix; Sanguisorba minor; Sanguisorba officinalis; Saxifraga; Selaginella; Sparganium/Typha; Sphagnum; Taxus; Thalictrum; Tilia; Ulmus; Valerianaceae; Viburnum
    Type: Dataset
    Format: text/tab-separated-values, 4680 data points
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