ISSN:
1420-9071
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Binding of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to adenylylated $$E_{\overline {11} } $$ glutamine synthetase is cooperative and time-dependent, with 3 dye sites per subunit. In fluorescence polarization experiments TNS and pyrene butyrate give normalized Perrin plots that indicate a symmetrical arrangement of dye excited state dipoles, relative to the rotational axis of the oblate ellipsoid of the dodecameric native enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01945941
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