ISSN:
1432-2048
Keywords:
Chlorella
;
Nitrate reductase (plasma-membrane bound)
;
Plasma membrane
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The plasma membranes of Chlorella saccharophila (Krüger) Nadson cells contained a membrane-bound nitrate reductase. This form of nitrate reductase was purified and characterized. Several differences from the soluble form of nitrate reductase were apparent, the most important being: (i) the greater hydrophobicity, as proven using Triton X-114 phase separation, hydrophobic interaction chromatography and stimulation by phosphilipids; (ii) the differences in the native molecular mass compared with Chlorella sorokiniana (Krüger) Nadson; and (iii) the different polypeptide pattern obtained by two-dimensional polyacrylamide gel electrophoresis. Only the plasma-membrane-bound nitrate reductase could be found in both inside-out and right-side-out plasma-membrane vesicles.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00240898
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