ISSN:
1420-9071
Keywords:
Immobilization
;
chicken liver fructose 1,6-biphosphatase
;
CNBr-activated Sepharose
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Chicken liver fructose 1,6-bisphosphatase is readily immobilized on CNBr-activated Sepharose. The immobilization alters some enzymatic properties. They include broader pH activity curve, loss of activation by K+ or NH 4 + , increased resistance to inactivation by trypsin, decreased sensitivity to AMP inhibition, and loss of cooperative interaction among AMP-binding sites. The immobilized enzyme retains about 38% or 19% of the specific activity of the native enzyme when the activity is measured in the absence or presence of K+, resepctively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01951702
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