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  • 1
    Electronic Resource
    Electronic Resource
    Apatite Formation on Porous HA/TCP in Animals’ Serums In Vitro (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 955-958 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Porous HA/TCP bioceramics were immersed in pure dog serum to observe apatiteformation. Deposited crystals were examined using SEM. Results showed that beamed sheet-likecrystals formed on the surface of ceramics granules, and after postponement immersion time, crystalsextended and became bigger. EDS and IR results suggested formed crystals were defect-calcium typecarbonated hydroxyapatite. HRTEM photograph suggested formation process of new-formedcrystals from non-crystal to crystal in serum. Directional organisms acted maybe as a template inprocess of crystals formation, so new crystals developed along certain direction
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.955.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation and Characterization of Nano Hydroxyapatite (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 235-238 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The aim of this study is to prepare nano hydroxyapatite powder. Hydroxyapatite powderwas prepared via co-precipitated method with the addition of citric acid at pH 9-11 in ambientenvironment. The precipitates were aged for 24hs, and then milled into powder after washed anddried. The particle morphology and particle size of as prepared HA powders were characterized.The results showed that hydroxyapatite powder with width of 10-30nm and length of 30-100nm wasprepared by wet co-precipitation
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.235.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of Different Hydroxyapatite Particles on the Behavior of Highly Malignant Melanoma Cells (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 279-282 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The aim of this study is to reveal the response of highly malignant tumor cell to differenthydroxyapatite particles. Human’s highly malignant melanoma (MM) cell line A875 is exposed totwo kinds of short-rod hydroxyapatite particles. After incubated with hydroxyapatite particles atdifferent time points, the effects of MM cell morphology, proliferation and invasion are evaluatedby SEM, MTT, and MMP2 protein immunohistochemistry respectively. SEM figures show thatsuperficial sentus of MM cells reduce; more secretion is found; and smaller HA particles have moreobvious effect. At the same time, no statistic difference can be found in MTT assay and MMP2expression measurement. This study also provides useful information about biosecurity ofhydroxyapatite ceramic in transplantation after highly malignant tumor excision
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.279.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    Quantitative Analysis of Core Binding Factor 1 (Cbfa1) and Osteocalcin in C2C12 Cells Induced by Ca/P Ceramics In Vitro (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1067-1070 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The mRNA expression of Cbfa1 and osteocalcin gene induced by calcium phosphateceramics (Ca/P) were quantitative analyzed according to real-time RT-PCR method in this work.C2C12 cells were co-culture with four kinds of porous Ca/P ceramics for 2 and 5d without addingother growth factors. The four kinds of Ca/P ceramics were pure hydroxyapatite (HA) sintered at1250ºC and HA/TCP with a ratio of 60/40 sintered at 1100ºC (HT1), 1200ºC (HT2) and 1250ºC(HT3) respectively. Real-time RT-PCR analysis found the Ca/P ceramics induced positiveexpression of Cbfa1 and osteocalcin in C2C12 cells, After 5 days culture, Cbfa1 and osteocalcinshowed obvious higher expression compared with that in 2 days. Cbfa1 and osteocalcin expressionin BCP was much higher than HA, and the expression level of osteocalcin wasHT1〉HT2〉HT3〉HA. Our results showed that Ca/P ceramics alone were sufficient to induceC2C12 cells to osteoblastic differentiation and the sinter temperature and phase composition ofCa/P ceramics could affect their osteoindctive capacity significantly
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1067.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    OPG and Rankl Expression in Osteoblasts Grown on Different HA Ceramics (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1095-1098 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Hydroxyapatite (HA) ceramics is one of the most widely used bone substitute in clinics.Limited information is available concerning how HA ceramics may affect osteoprotegerin (OPG)and RANKL expression. In this study, osteoblastic-like SaOS-2 cells were grown on HA ceramicssintered at different temperature for 3 and 6 days, RANKL and OPG mRNA expression wereanalyzed with quantitative in situ hybridization (QISH) technique. Result showed that SaOS-2grown on HA ceramics sintered at 800℃ expressed higher RANKL mRNA than on other two HAceramics after 3 days’ culture. No significant difference in OPG expression on different surfaceswas detected after 3 days and 6 days culture. This result suggests that HA sintered at lowtemperature tend to induce more bone remodeling after implantation
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1095.pdf
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  • 6
    Electronic Resource
    Electronic Resource
    Constructing Bone Graft with Calcium Phosphate Ceramics in Different Non-Osseous Tissue (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 641-644 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The study is aimed at to explore the construction of bone graft with calcium phosphateceramics implanted in subcutaneous fat, and provide applying technique for the in vivo bone tissueengineering. Forty-eight calcium phosphate ceramics (Ca-P ceramics) columns with Φ 5×8 mmwere prepared, and eight dogs were used in this experiment. Six samples were implanted in eachdog. Three in dorsal muscles while the others in subcutaneous fat. The specimens were harvested at4, 6, 12 and 24 weeks post operation, for gross observation, SPECT and histological studies. Theosseous or osteoid tissue formation at different times in the two non-osseous sites was comparedand the new bone grafts in subcutaneous fat and intramuscular implantations were evaluated. Theresults demonstrated that the bone graft could be constructed not only in muscle but also insubcutaneous fat. Compared to that in muscle, constructing bone graft in subcutaneous fat couldhave brighter prospect to clinical application
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.641.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of Different Hydroxyapatite Particles on Malignant Melanoma Cell Behavior (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 761-764 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The effect of different hydroxyapatite particles on malignant melanoma cell wasevaluated in vitro. Two kinds of short-rode hydroxyapatite particles, which range from 0.4 to 1 umand 0.5 to 1.5 um, were co-cultured with human’s malignant melanoma (MM) cell line A375 for 24,48, and 72 h. Both of these HA particles showed the same cell proliferation rate as blank control,and there was no statistically significant correlation of matrix metalloproteinases-2 (MMP-2)expression to the HA particle size. SEM images showed that the membrane of tumor cell, whichco-cultured with HA particles, was shrunk. The cell superficial sentus was reduced and small sizeparticle’ effect was more obvious. Since immunohistochemistry and SEM data are qualitativetechnique, further precise methods might bring more information about the effect of HA particles ontumor behaviors. But this study can provide the bio-security test of micrometer HA particles intransplantation after tumor excision
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.761.pdf
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  • 8
    Electronic Resource
    Electronic Resource
    Preparation of Nano-Hydroxyapatite/Poly(D,L-Lactide) Composite and Its Degradation in Femora of Rabbits (2005)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 288-289 (June 2005), p. 203-206 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: With the outstanding biocompatibility of hydroxyapatite (HA) and biodegradation ofpoly(D,L)lactide(PDLLA), and the expected good bio-mechanical compatibility, nano-HA / PDLLA (n-HA/PDLLA)composite has been paid great interests in hard tissue repair. One of the key factors affecting the potential of the composite is the degradation of the composite. That is what the mechanism of degradation in the composite is and if the degradation of the materials would induce the crack of the composite or a porous structure facile for tissue ingrowth would be formed. In this study, an n-HA/ PDLLA composite containing about 40% n-HA (wt%) was prepared and the degradation of the composite in bony tissue of rabbits and tissue response were studied by implanting composite rods and control HA rods into the femora of 16 New Zealand rabbits. After definite intervals, the histological analysis was completed by light microscopy and the degradation behavior was observed by scanning electron microscopy. The results suggested that a nano-HA/PDLLA composite was obtained and the materials showed good biocompatibility andosteoconductivity. The substantial degradation of the composite occurred at 8 weeks in vivo. After a longer period of implantation, the further degradation of the composite led to the formation of interconnected microporous and macroporous structure in the materials that might facilitate the tissue ingrowth in the composite
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/49/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.288-289.203.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    An Improvement in Sintering Property of β-Tricalcium Phosphate by Addition of Calcium Pyrophosphate and Calcium Carbonate (2005)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 475-479 (Jan. 2005), p. 2359-2362 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: b-tricalcium phosphate(TCP, Ca3(PO4)2) ceramics are preferred as a bioceramics because of its chemical stability and reasonable degradation rate in vivo, but it is difficult to obtain b-TCP ceramics with high compressive strength at lower temperature than that of phase transition to a-TCP. In this study, the sintering behavior of TCP, Ca2P2O7-doped TCP, and CaCO3-doped TCP in the range of 2wt%~5wt% were investigated respectively. Phase transition of pure TCP took place between 1100℃ to 1150℃, and pure b-TCP ceramics could achieve acompressive strength of only 3MPa. However, calcium pyrophosphate (CPP, Ca2P2O7) additive prevented the transformation of b-TCP to a-TCP, but the second phase of CPP was observed in the resultant ceramics. Phase transition of TCP ceramics by addition of both CPP and calcium carbonate (CC, CaCO3) took place between 1200℃ to 1250℃ and the resultant TCP ceramics had few impurity of CPP. By adding CPP and CC to TCP, final ceramics with compressive strength over 12MPa could be obtained when sintered at 1200℃ for 2hrs
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/09/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.475-479.2359.pdf
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  • 10
    Electronic Resource
    Electronic Resource
    Fabrication of Porous Hydroxyapatite Ceramics by Microwave Sintering Method (2005)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 288-289 (June 2005), p. 529-532 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Microwave processing of porous hydroxyapatite ceramics was investigated in a dualfrequency microwave-sintering furnace. The results revealed that microwave-sintering process could get sintered ceramics at much shorter sintering time and at lower sintering temperature than that of the conventionally heat-sintering process. Further, the microwave-sintered samples showed much smaller grain size and more uniform microstructure and reached a comparable compressive strength. The mechanism of rapid heating behavior of hydroxyapatite ceramics in microwave was also discussed. The results revealed that microwave processing was a promising method for sintering porous hydroxyapatite ceramics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/49/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.288-289.529.pdf
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