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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    The @Journal of Steroid Biochemistry and Molecular Biology 50 (1994), S. 253-260 
    ISSN: 0960-0760
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Plasmid 10 (1983), S. 199-203 
    ISSN: 0147-619X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Pesticide Biochemistry and Physiology 39 (1991), S. 93-99 
    ISSN: 0048-3575
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 46 (1974), S. 11-16 
    ISSN: 0014-5793
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 24 (1986), S. 477-486 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Protocols for the isolation of cellulolytic actinomycetes are described, and their use illustrated in the selection of thermophilic bacteria from soil. One isolate, Microbispora bispora, was selected for further study. It grew readily at 55°C, produced an extracellular cellulase in good yield (endoglucanase, 5.9 U/ml) that had a broad pH range (pH 5.5–7.2) and was thermally stable. Its aryl-β-glucosidase was cell-associated and was relatively resistant to end-product inhibition.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 24 (1986), S. 487-492 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The saccharification efficiency of cellulase from the thermophilic actinomycete Microbispora bispora was evaluated using commercially available feedstocks. The enzyme preparation was effective against refuse derived cellulose with 30% being converted to glucose in a 24 hour period. Pretreatment of the refuse with cadoxen resulted in an increase in saccharification efficiency to 70%.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 5 (1985), S. 103-108 
    ISSN: 1573-5028
    Keywords: binary vector ; hygromycin B resistance ; plant transformation marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A bacterial gene encoding hygromycin phosphotransferase has been modified for expression in tobacco cells. The aphIV gene from Escherichia coli was inserted between the 5′ sequence of an octopine synthase gene and the 3′ sequence from a nopaline synthase gene. The new gene was incorporated between T-DNA border fragments in the broad-host-range vector pKT210 to form a micro-Ti plasmid. Agrobacterium tumefaciens containing this plasmid and a Ti plasmid as helper was used to incite crown gall tumors on aseptic tobacco plants. Samples of these galls could grow in the presence of hygromycin B, provided that the aph gene had been fused with the ocs gene to maintain the sense of the coding sequences. When the genes had been fused in the reverse ‘antisense’ orientation none of the gall samples could grow on hygromycin. Unlike wild-type galls the hygromycin-resistant tissue contained DNA sequences homologous to the aphIV gene. Thus the modified gene can be introduced into tobacco cells and confer on them the ability to grow in the presence of hygromycin B.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 134 (1974), S. 99-113 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutant strains of Aspergillus nidulans have been isolated which grow normally on minimal medium at 37°C but not at 20°C. Growth tests indicate that these seventy-five mutant strains (designated CS, cold-sensitive) have a range of defects. Five are auxotrophic at 20°C, one (CS13) requiring isoleucine and another (CS48) choline. Many mutants are osmotic remedials. Some CS strains have altered properties at 37°C, including deoxycholate-sensitivity, actidione-resistance or actidione-ultrasensitivity. The majority of thirty-two CS strains tested segregate cold-sensitivity as single gene mutations in crosses with wild-type. Cold-sensitivity of one strain (CS67) is cytoplasmically inherited. Dominance tests in heterokaryons showed that in each of twenty-six CS strains examined cold-sensitivity is determined by recessive mutations (designated cs). Complementation analysis of nine cs mutations showed that they each affect a different function. Crosses between some cs mutants, and the allocation of other cs mutations to different linkage groups, demonstrate that mutations to cold-sensitivity are not restricted to a limited region of the genome. These results indicate that in Aspergillus nidulans selection for cold-sensitivity provides an enrichment for mutants with alterations in many different cellular properties.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 134 (1974), S. 115-132 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four cold-sensitive mutants of Aspergillus nidulans which produce abnormal ribosome sedimentation profiles have been identified. After growth at 37° C followed by incubation at 20° C, all four mutant strains (designated ARP) produce profiles containing a reduced ratio of large to small ribosomal subunits. In at least three of the ARP strains the altered profile results from decreased production of the large ribosomal subunit at the non-permissive temperature. No mutants were found to accumulate incomplete ribosomal precursors under these conditions. In each ARP strain there is a single mutation which determines both cold-sensitivity and production of an altered ribosome profile. These four mutations define three loci. The arpA locus is in linkage group VII. The arpB and arpC loci are far apart in linkage group VIII. Two additional unlinked mutations, one determining actidione-ultrasensitivity and the other cold-sensitivity, were identified in the ARP strains. Both mutations at the arpA locus also confer resistance to a high concentration of actidione (2 mg ml-1). Additional actidione-resistant, cold-sensitive strains were isolated but none had the ARP phenotype. Recombination analysis of actidione resistant mutants has identified six new loci determining resistance to the drug in A. nidulans.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 2091-2092 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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