ISSN:
1432-0878
Keywords:
Concanavalin A
;
Bone marrow
;
Differentiation
;
Cell surface
;
Ultrastructural cytochemistry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The surface characteristics of hemopoietic cells in normal human bone marrow have been explored by the use of the ultrastructural concanavalin A-peroxidase-diaminobenzidine (CAPD) procedure for the detection of specific carbohydrate residues (α-D-mannopyranoside, α-D-glucopyranoside, and α-N-acetyl-D-glycosaminide) associated with the cell surface. All cells present in the bone marrow were capable of binding Con A to their surfaces. The extent of binding proved cell specific and could be related to the stage of morphological development of each cell line. Maximum surface reactivity in the bone marrow cell population occurred with the most immature cells (myeloblast and erythroblast), lymphocytes, eosinophils, monocytes, macrophages and platelets, while mature neutrophils, basophils, and erythrocytes showed only minimum surface reactivity. These findings serve not only to expand our knowledge of the chemical nature of the surface of differentiating hemopoietic cells but provide striking evidence that modifications in the surface of hemopoietic cells occur during the process of normal cell differentiation in the bone marrow.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00220141
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