ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Raw green snapbeans (Phaseolus vulgaris var. Slendergreen) were cut, frozen, lyopbilized, and extracted with chloroform-methanol 2:l. The crude extract amounted to 9.9% of the dry weight of the beans. The lipid material in the extract composed 2.6% of the dry weight of the bean. The crude lipids were fractionated with acetone, and the acetone-soluble portion was subjected to countercurrent distribution between n-heptane and 95% methanol. The major lipid fractions were treated with glacial acetic acid, followed by partition between benzene and 50% aqueous acetic acid to aid in removal of non-lipid contaminants. The composition of snapbean lipids was studied by measuring the nitrogen, phosphorus, sugar, and fatty acid content of the various fractions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1963.tb00232.x
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