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  • 1
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    Sodium-selective liquid ion-exchanger microelectrodes for intracellular measurements (1979)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1979-03-30
    Description: The sodium-selective ligand 1,1,1-tris[1(1)-(2(1)-oxa-4(1)-oxo-5(1)-aza-5(1)-methyl)dodecanyl]propane dissolved in 3-nitro-o-xylene containing a small amount of the lipophilic anion tetrachlorophenyl borate was used as a liquid ion-exchanger in sodium-selective microelectrodes. The microelectrodes gave rapid, stable responses that were linear functions of the logarithm of sodium activity. They were tested under conditions approximating those to be expected in the cell interior, and the results indicated that they can be used to measure intracellular sodium activity without significant interference from intracellular potassium.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉O'Doherty, J -- Garcia-Diaz, J F -- Armstrong, W M -- New York, N.Y. -- Science. 1979 Mar 30;203(4387):1349-51.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/424756" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cytoplasm/*analysis ; Intestinal Mucosa/analysis ; Ionophores ; Ligands ; *Microelectrodes ; Potassium/analysis ; Sodium/*analysis ; Urodela
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Calcium regulation during stimulus-secretion coupling: continuous measurement of intracellular calcium activities (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-07-25
    Description: Accurate measurements of intracellular calcium activities in salivary gland epithelial cells of the insect Phormia regina were obtained with microelectrodes in which N,N'-di(11-ethoxycarbonyl)undecyl-N,N'-4,5-tetramethyl-3,6-dioxaoctane diacid diamide wsa incorporated in a liquid membrane system. When calibrated in solutions approximating the ionic concentration of the cell interior, these microelectrodes gave rapid stable responses that were linear functions of the logarithm of calcium activities and were not affected by potassium, sodium and magnesium. Continuous monitoring of calcium activities during serotonin-induced saliva release provided direct evidence of hormonal influence on transmembrane calcium movement and spontaneous regulation of intracellular calcium by stimulated cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉O'Doherty, J -- Youmans, S J -- Armstrong, W M -- Stark, R J -- New York, N.Y. -- Science. 1980 Jul 25;209(4455):510-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7394518" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Transport/drug effects ; Calcium/*metabolism ; Diptera/*metabolism ; Epithelium/metabolism ; Kinetics ; Magnesium/pharmacology ; Microelectrodes ; Salivary Glands/drug effects/*metabolism ; Serotonin/pharmacology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
    Electronic Resource
    Electronic Resource
    Cyclic AMP-induced changes in membrane conductance ofNecturus gallbladder epithelial cells (1985)
    Springer
    The journal of membrane biology 84 (1985), S. 193-206 
    Details
    ISSN: 1432-1424
    Keywords: Necturus gallbladder ; apical membrane potentials ; intracellular chloride activities ; apical membrane conductances ; basolateral membrane K+ conductance ; double-barreled Cl−-selective microelectrodes ; IBMX
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Enhanced cellular cAMP levels have been shown to increase apical membrane Cl− and HCO 3 − conductances in epithelia. We found that the phosphodiesterase inhibitor 3-isobutyl-1-methyl-xanthine (IBMX) increases cAMP levels inNecturus gallbladder. We used conventional open-tip and double-barreled Cl−-selective microelectrodes to study the effects of IBMX on membrane conductances and intracellular Cl− activities in gallbladders mounted in a divided chamber and bathed with Ringer's solutions at 23°C and pH 7.4. In HCO 3 − -free media, 0.1mM IBMX added to the mucosal medium depolarized the apical membrane potentialV a , decreased the fractional resistanceF R , and significantly reduced intracellular Cl− activity (a Cl i ). Under control conditions,a Cl i was above the value corresponding to passive distribution across the apical cell membrane. In media containing 25mM HCO 3 − , IBMX caused a small transient hyperpolarization ofV a followed by a depolarization not significantly different from that observed in HCO 3 − -free Ringer's. Removal of mucosal Cl−, Na+ or Ca2+ did not affect the IBMX-induced depolarization inV a . The basolateral membrane ofNecturus gallbladder is highly K+ permeable. Increasing serosal K+ from 2.5 to 80mM, depolarizedV a . Mucosal IBMX significantly reduced this depolarization. Addition of 10mM Ba2+, a K+ channel blocker, to the serosal medium depolarizedV a and, essentially, blocked the depolarization induced by IBMX. These results indicate that mucosal IBMX increases apical HCO 3 − conductance and decreases basolateral K+ conductance in gallbladder epithelial cells via a cAMP-dependent mechanism. The latter effect, not previously reported in epithelial tissues, appears to be the major determinant of the IBMX-induced depolarization ofV a .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871383
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