Publication Date:
2001-08-04
Description:
An early event in RNA interference (RNAi) is the cleavage of the initiating double-stranded RNA (dsRNA) to short pieces, 21 to 23 nucleotides in length. Here we describe a null mutation in dicer-1 (dcr-1), a gene proposed to encode the enzyme that generates these short RNAs. We find that dcr-1(-/-) animals have defects in RNAi under some, but not all, conditions. Mutant animals have germ line defects that lead to sterility, suggesting that cleavage of dsRNA to short pieces is a requisite event in normal development.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855227/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855227/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Knight, S W -- Bass, B L -- R01 GM044073/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Sep 21;293(5538):2269-71. Epub 2001 Aug 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Howard Hughes Medical Institute, University of Utah, 50 North Medical Drive, Room 211, Salt Lake City, UT 84132, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11486053" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Genetically Modified
;
Caenorhabditis elegans/cytology/*enzymology/*genetics/growth & development
;
Cell Differentiation
;
Disorders of Sex Development
;
Endoribonucleases/genetics/*metabolism
;
Female
;
*Gene Silencing
;
Genes, Helminth
;
Germ Cells/*cytology/metabolism
;
Male
;
Mutation
;
Oocytes/cytology
;
Phenotype
;
RNA, Double-Stranded/*genetics/*metabolism
;
RNA, Helminth/genetics/metabolism
;
RNA, Messenger/genetics/metabolism
;
Reverse Transcriptase Polymerase Chain Reaction
;
Ribonuclease III
;
Sequence Deletion
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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