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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of a carotenoid biosynthesis gene coding for ζ-carotene desaturase from Anabaena PCC 7120 by heterologous complementation (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 106 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A screening procedure for carotenoid genes involving heterologous complementation with two different plasmid constructs was developed. The plasmids contained the crtE and crtB genes from Erwinia unredovora together with the phytoene desaturase gene from either Rhodobacter capsulatus or Synechococcus PCC 7942. Transformation in E. coli led to the accumulation of neurosporene and ζ-carotene, respectively. Co-transformation with an Anabaena plasmid library resulted in the isolation of the two plasmids, pZDS1 and pZDS1. Their gene products showed the ability to convert neurosporene and ζ-carotene into lycopene. In contrast, accumulated phytoene could not be converted. We conclude that the cloned gene codes for the carotenoid biosynthesis gene ζ-carotene desaturase (zds).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb05941.x
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  • 2
    Electronic Resource
    Electronic Resource
    Trans-translation and protein synthesis inhibitors (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 218 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Trans-translation is a process found in all bacteria, which contributes to the release of ribosomes that are stalled through a variety of causes, for example when the 3′ end of a truncated mRNA lacking a stop codon is reached or at internal clusters of rare codons. Trans-translation requires tmRNA. Trans-translation is not essential for cell viability under laboratory conditions, but recently it has been shown that it can contribute to cell viability in the presence of protein synthesis inhibitors. In this minireview, we consider the connection between trans-translation and antibiotics and the potential of using trans-translation as a therapeutic target.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2003.tb11491.x
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  • 3
    Electronic Resource
    Electronic Resource
    Modification of 40S ribosomal subunits from yeast with dimethylmaleic anhydride (1986)
    Springer
    Molecular biology reports 11 (1986), S. 137-141 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Modification of 40S ribosomal subunits from Saccharomyces cerevisiae with dimethylmaleic anhydride (DMMA Abbreviation: DMMA, 2,3-dimethylmaleic anhydride. ), a reagent for protein amino groups, is accompanied by loss of polypeptide-synthesizing activity and by dissociation of proteins from the particles. The protein-deficient ribosomal particles, originated from 40S subunits by treatment with dimethylmaleic anhydride at a molar ratio of reagent to particle of 250, can partially reconstitute active subunits upon addition of the corresponding released proteins, and regeneration of the modified amino groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419733
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  • 4
    Electronic Resource
    Electronic Resource
    RNase P from bacteria. Substrate recognition and function of the protein subunit (1995)
    Springer
    Molecular biology reports 22 (1995), S. 99-109 
    Details
    ISSN: 1573-4978
    Keywords: catalytic RNA ; RNase P ; tRNA ; precursors of tRNAs ; protein-RNA interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RNase P recognizes many different precursor tRNAs as well as other substrates and cleaves all of them accurately at the expected position. RNase P recognizes the tRNA structure of the precursor tRNA by a set of interactions between the catalytic RNA subunit and the T- and acceptor-stems mainly, although residues in the 5′-leader sequence as well as the 3′-terminal CCA are important. These conclusions have been reached by several studies on mutant precursor tRNAs as well as cross-linking studies between RNase P RNA and precursor tRNAs. The protein subunit of RNase P seems also to affect the way that the substrate is recognized as well as the range of substrates that can be used by RNase P, although the protein does not seem to interact directly with the substrates. The interaction between the protein and RNA subunits of RNase P has been extensively studiedin vitro. The protein subunit sequence is not highly conserved among bacteria, however different proteins are functionally equivalent as heterologous reconstitution of the RNase P holoenzyme can be achieved in many cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988713
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  • 5
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of the phytoene desaturase gene from Synechocystis sp. PCC 6803 and characterization of a new mutation which confers resistance to the herbicide norflurazon (1992)
    Springer
    Plant molecular biology 18 (1992), S. 981-983 
    Details
    ISSN: 1573-5028
    Keywords: carotenogenesis ; herbicide resistance ; norflurazon ; phytoene desaturase ; Synechocystis sp. PCC 6803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019213
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  • 6
    Electronic Resource
    Electronic Resource
    A higher-plant type ζ-carotene desaturase in the cyanobacterium Synechocystis PCC6803 (1998)
    Springer
    Plant molecular biology 36 (1998), S. 725-732 
    Details
    ISSN: 1573-5028
    Keywords: ζ-Carotene desaturase gene ; J852 ; lycopene ; neurosporene ; phylogeny ; Synechocystis PCC6803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genomic DNA sequence of Synechocystis was analysed for putative ζ-carotene desaturase genes. Two promising candidates slr0940 and slr0033 were found with similarities to the structurally different ζ-carotene desaturase genes from higher plants and Anabaena, respectively. Only the expression product of the analogue to the plant gene, slr0940, was able to mediate the 2-step desaturation of ζ-carotene via neurosporene to lycopene after complementation of this pathway in Escherichia coli. When enzyme reactions were carried out with this protein, activity was obtained with either ζ-carotene or neuroporene as substrates. The in vitro reaction was inhibited by the pyrimidine derivative J852 which is effective as experimental herbicide in plants. The occurrence of two different types of ζ-carotene desaturases among cyanobacteria and the phylogenetic consequences on chloroplast evolution are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005997405431
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  • 7
    Electronic Resource
    Electronic Resource
    Partial reconstitution of 60S ribosomal subunits from yeast (1985)
    Springer
    Molecular and cellular biochemistry 66 (1985), S. 55-60 
    Details
    ISSN: 1573-4919
    Keywords: dimethylmaleic anhydride ; ribosome reconstitution ; 60S ribosomal subunits ; yeast ribosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Ribosomal 60S subunits active in polyphenylalanine synthesis can be reconstituted from core particles lacking 20–40% of the total protein. These core particles were obtained by treatment of yeast 60S subunits with dimethylmaleic anhydride, a reagent for protein amino groups. Upon reconstitution a complementary amount of split proteins is incorporated into the ribosomal particles, which have the sedimentation coefficient of the original subunits. Ribosomal protein fractions obtained by extraction with 1.25 M NH4Cl, 4 M LiCl, 7 M LiCl, or 67% acetic acid, are much less efficient in the reconstitution of active subunits from these core particles than the corresponding released fraction prepared with dimethylmaleic anhydride. Attempts to reconstitute active subunits from protein-deficient particles obtained with 1.25 M NH4Cl plus different preparations of ribosomal proteins, including the fraction released with dimethylmaleic anhydride, were unsuccessful. Therefore, under our conditions, of the disassembly procedures assayed only dimethylmaleic anhydride allows partial reconstitution of active 60S subunits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231823
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  • 8
    Electronic Resource
    Electronic Resource
    Involvement of lysine and arginine residues in the binding of yeast ribosomal protein YL3 to 5S RNA (1987)
    Springer
    Molecular and cellular biochemistry 76 (1987), S. 141-146 
    Details
    ISSN: 1573-4919
    Keywords: ribosomal protein YL3 ; 5S RNA ; lysine residues ; arginine residues ; yeast ribosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The contribution of lysine and arginine residues to the formation of yeast ribonucleoprotein complex 5S RNA. protein YL3 has been investigated by determining the effects on complex formation of modification with chemical reagents specific for either lysine or arginine. Treatment of protein YL3 with acetic anhydride, malefic anhydride or phenylglyoxal is accompanied by loss of its capacity to bind to 5S RNA. This effect is accomplished by modification with phenylglyoxal of only 3 arginine residues per YL3 molecule. In contrast, a large number of protein YL3 amino groups [16] must be modified by acetic anhydride to prevent complex formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223479
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  • 9
    Electronic Resource
    Electronic Resource
    Detection of soluble peroxidases by direct plant tissue isoelectric focusing (1986)
    Weinheim : Wiley-Blackwell
    Electrophoresis 7 (1986), S. 392-393 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Rectangular sections of leaves, vacuum-infiltrated with distilled water or aqueous solutions of potassium chloride, were applied directly onto the surface of polyacrylamide gels followed by isoelectric focusing. Soluble peroxidases (free and ionically bound) were released from the tissue on isoelectric focusing with resultant characteristic zymograms for the different plant tissues.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150070811
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  • 10
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    Enzymatic cleavage of RNA by RNA (1986)
    Cell Press
    Details
    Publication Date: 1986-12-01
    Print ISSN: 0968-0004
    Electronic ISSN: 1362-4326
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Published by Cell Press
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