Publication Date:
2013-08-10
Description:
Chromosome translocations are a hallmark of cancer cells. We have developed an experimental system to visualize the formation of translocations in living cells and apply it to characterize the spatial and dynamic properties of translocation formation. We demonstrate that translocations form within hours of the occurrence of double-strand breaks (DSBs) and that their formation is cell cycle-independent. Translocations form preferentially between prepositioned genome elements, and perturbation of key factors of the DNA repair machinery uncouples DSB pairing from translocation formation. These observations generate a spatiotemporal framework for the formation of translocations in living cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roukos, Vassilis -- Voss, Ty C -- Schmidt, Christine K -- Lee, Seungtaek -- Wangsa, Darawalee -- Misteli, Tom -- Intramural NIH HHS/ -- New York, N.Y. -- Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Cancer Institute, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23929981" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bacterial Proteins/genetics
;
Carrier Proteins/genetics
;
Cell Cycle
;
*DNA Breaks, Double-Stranded
;
DNA Repair
;
DNA-Activated Protein Kinase/antagonists & inhibitors
;
DNA-Binding Proteins/antagonists & inhibitors
;
Green Fluorescent Proteins/genetics
;
High-Throughput Screening Assays
;
Lac Operon
;
Lac Repressors/genetics
;
Mice
;
Microscopy/methods
;
NIH 3T3 Cells
;
Neoplasms/genetics
;
Nuclear Proteins/antagonists & inhibitors
;
*Time-Lapse Imaging
;
*Translocation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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