ISSN:
0006-3592
Keywords:
insect cells
;
microfiltration
;
hollow fiber
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
An efficient method was developed for media separation and cell collection for eukaryotic cells growing in suspension. The method is based on tangential flow microfiltration using an open channel arrangement in a hollow fiber configuration. Best results (highest processing flux rate) for polysulfone hollow fibers were obtained using fibers with internal diameter of 0.75 mm, 0.45 μm pore size, and a cell suspension flow at a shear rate of 14000 s-1 (0.032 L/min per fiber). A flux rate of 500 L/m2 h can be obtained by maintaining the surface area/cell ratio at 0.05 m2/10 L of cells at a concentration of 2.5 × 106 cells/mL. Forty liters of infected insect cells can be concentrated 10 times in 20 min without affecting cell viability. © 1995 John Wiley & Sons, Inc.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260480412
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