ISSN:
0173-0835
Keywords:
Capillary electrophoresis
;
Neutral surfactants
;
Polyelectrolytes
;
Amphiphilic polymers
;
Polymer-surfactant interactions
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Hydrophobically modified poly(acrylic acids) (HMPAs) are random copolymers of sodium acrylate and dodecyl acrylamide, containing 0-10% mol/mol of dodecyl grafts. The hydrophobic character of different HMPAs of average molecular weight 150 000 was studied by capillary electrophoresis (CE), using neutral surfactants as buffer additives. The differentiation of the electrophoretic mobilities of HMPAs with their hydrophobicity was achieved through the use of nonionic Brij 35 and zwitterionic DAPS surfactants. A nearly baseline separation of the precursor and three HMPAs derivatives was obtained in a poly(ethylene glycol)-coated capillary with a background electrolyte containing 10 mM N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (DAPS) and 10 mM borax (pH 9.2). In addition to CE experiments, the polymer-surfactant interactions were also investigated by means of quasi-elastic light scattering (QELS) and viscosimetric measurements. According to the latter results, the separation mechanism was interpreted as an expansion of the polymer coil in the presence of micelles and subsequent change of its frictional properties. A true micellar electrokinetic capillary chromatography (MEKC) partitioning model was discarded on the basis of the relative sizes of the macromolecule and the micelles.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150170706
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