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  • 1
    Electronic Resource
    Electronic Resource
    Mutation and inactivation of mammalian cells by various ionising radiations (1977)
    [s.l.] : Nature Publishing Group
    Nature 267 (1977), S. 425-427 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] LET is a measure of the deposition of energy along the tracks of the charged particles produced in irradiated material. The distribution of energy loss events along a track depends on the type of radiation; events are widely-spaced for radiations of low LET such as X or 7 rays, but more closely ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/267425a0
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  • 2
    Electronic Resource
    Electronic Resource
    Non-rejoining DNA breaks and cell inactivation (1978)
    [s.l.] : Nature Publishing Group
    Nature 272 (1978), S. 379-380 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] RITTER et al.1 measured non-rejoining DNA strand breaks (NRBs) produced in V79 hamster cells by radiations of different linear energy transfer (LET) and found a correlation between the efficiencies of NRB production and low-dose 'single-hit' cell inactivation. However, we suggest that this ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/272379a0
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  • 3
    Electronic Resource
    Electronic Resource
    Mutation induction and inactivation in mammalian cells exposed to ionising radiation (1975)
    [s.l.] : Nature Publishing Group
    Nature 258 (1975), S. 429-431 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Survival curves of irradiated human diploid and V79 Chinese hamster cells. Closed symbols, human cells; open symbols, hamster cells. Radiations: A V A 60Co y rays; o 250 keV X rays; D 5 keV unr1 protons. HF19 human diploid fibroblasts were initiated from female embryonic lung tissue and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/258429a0
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  • 4
    Electronic Resource
    Electronic Resource
    Mutations induced by DNA double-strand breaks: The influence of genomic site (1996)
    Springer
    Somatic cell and molecular genetics 22 (1996), S. 249-259 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The transgenic CHO cell line PL61, carrying a recombinant SV40-gpt gene, was treated with restriction endonucleases to assess mutagenesis from defined DNA double-strand breaks. Mutations ingpt were measured under two conditions: a stringent condition where selection ensured that the closely-linkedneo gene was retained functionally intact, or a relaxed condition without the requirement forneo gene function. Despite testing 18 different restriction endonucleases with various numbers of potential break-sites within the transgene, mutations were only found under relaxed selection conditions. These mutations commonly led to complete loss of the transgene, suggesting that large deletions predominate when selection is relaxed. It is argued, in comparison to mutation data for other genomic sites in CHO cells, that variations in the ‘effective target size’ for mutagenesis may explain the response of the transgene under different conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02369565
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  • 5
    Electronic Resource
    Electronic Resource
    Analysis of large deletions in theHPRT gene of primary human fibroblasts using the polymerase chain reaction (1993)
    Springer
    Somatic cell and molecular genetics 19 (1993), S. 9-19 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Spontaneous and X-ray-induced mutants of theHPRT gene were isolated from two primary human fibroblast lines. The limited life-span of the mutants restricted the use of methods requiring large quantities of DNA, and the polymerase chain reaction (PCR) was used in particular to check for the presence of multiple genomic sites in mutant analysis. Robust PCR primers were designed to amplify sites of up to 1 kb, mostly with 1-kb spacings between sites, over the entire 56-kbHPRT gene region. Using PCR, large deletions were found in 43% of independent X-ray-induced mutants, and their breakpoints were localized where these fell within the gene. Anonymous DNA sites in the Xq26 chromosomal region containingHPRT (covering ≥1.5 Mb) were also amplified by PCR to assess codeletion withHPRT; sites up to 1 Mb distal to the gene (DXS86, DXS10) were codeleted in some mutants, but no mutant was found with loss of a proximal site (DXS79).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01233950
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  • 6
    Electronic Resource
    Electronic Resource
    Fingerprinting cell lines: Use of human hypervariable DNA probes to characterize mammalian cell cultures (1988)
    Springer
    Somatic cell and molecular genetics 14 (1988), S. 519-525 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Hypervariable DNA sequences may be used as probes to derive DNA “fingerprints” for individuals. To assess the use of the human 33.15 and 33.6 probes (isolated by Jeffreys and coworkers) for characterizing cell lines of nonhuman origin, DNA from different stocks of Chinese hamster (CH) cells was screened. All CHO (ovary) sublines could be readily distinguished from CH-V79 sublines by their fingerprints, but where two stocks had been derived recently from the same line, their fingerprints were nearly identical. Similarly fingerprints of HPRT-deficient mutants derived from one cell stock were identical. A V79 × CHO fusion hybrid showed equal fingerprint band-sharing with each parent line, while early-passage diploid CH cells had a fingerprint closer to CHO than to V79. Thus these data introduce a simple means of typing cell lines to avoid cross-contamination, of checking cell hybrids, and of assessing the divergence of cell stocks from one another.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01535307
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  • 7
    Electronic Resource
    Electronic Resource
    The effectiveness of restriction endonucleases in cell killing and mutation (1993)
    Springer
    Somatic cell and molecular genetics 19 (1993), S. 479-490 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The use of restriction endonucleases (RE) to study the importance of DNA break end structures in differential cellular response has proved controversial. The number of DNA cut sites and the accessibility of RE are recognized examples of confounding factors. We have eliminated these factors by comparing the effectiveness of isoschizomers. Additionally, we considered for the first time the tolerance of the enzymes to cellular conditions. Cell killing and mutation were compared to the overall cutting ability of the enzymes in an “intracellular” buffer. We found that the activity of each RE combined with its lifetime, under simulated cellular conditions, were the overriding factors in determining effectiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01233253
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  • 8
    Electronic Resource
    Electronic Resource
    Inherited sensitivity to X-rays in man (1989)
    New York, NY : Wiley-Blackwell
    BioEssays 11 (1989), S. 58-62 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ataxia-telangiectasia (A-T), an inherited disorder giving radiation sensitivity and cancer-proneness, is discussed in terms of a defect in ability to repair DNA damage. A new assay using damaged recombinant DNA molecules suggests that the fidelity of repair of DNA double-strand breaks is reduced in an A-T cell line. Specific chromosomal changes in some A-T patients appear to be associated with cancer induction, and it is suggested that these could be linked to a DNA repair-fidelity defect. However, a general correlation between radiosensitivity and cancer-proneness is difficult to establish at present, partly because of diversity in radiosensitivity in the normal population.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950110205
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  • 9
    Electronic Resource
    Electronic Resource
    Influence of needle holder jaw configuration on the biomechanics of curved surgical needle bendingThis research was supported by a generous gift from the Texaco Philanthropic Foundation, White Plains, New York. (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 1 (1990), S. 39-47 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770010107
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  • 10
    Electronic Resource
    Electronic Resource
    A synthetic membrane for testing needle penetration (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 4 (1993), S. 157-160 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Needle performance is primarily judged by its penetration through tissue. For detailed studies of needle performance, tissue is too heterogeneous and unstable and difficult to obtain. A homogeneous, stable, and readily available polyurethane substrate has been evaluated as a suitable alternative to biological tissue for evaluation of needle penetration. The needle penetration profiles through the synthetic substrate correlated very well with those through natural tissue. In addition, the synthetic substrate was sensitive enough to easily detect a 20% increase in the force necessary to penetrate natural tissue. This polyurethane substrate offers a valid alternative to biological tissue for the testing of needle penetration performance. © 1993 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770040207
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