ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
A big variety of bioceramics have been successfully utilized as implant materials forpromoting fixation of bony tissues. Different bioceramics exhibited markedly different proliferationrates of the osteoblast cells in vitro. Clarification of the mechanism about the attachment andproliferation/differentiation of the cells would contribute to selecting suitable biomaterials for hardtissue replacement. Proteomics study was performed in this study employing the 2-dimensionalelectrophoresis assay with an aim of recognizing the changes in proteins. Nanostructuredhydroxyapatite (HA) coatings have been fabricated and they have shown promising mechanicalperformances. Results showed that the nanostructured HA coatings promoted proliferation of theosteoblast cells. Alkaline phosphatase (ALP) assay revealed an increased ALP activity of theproliferated viable cells, and obviously the presence of the nanosized pores can enhance theanchoring and stretching of the cells. No obvious difference in the 2-D gel maps taken for the cellsproliferated on the HA coating and for control can be found. This in turn suggests that thenanostructured HA coating induces minor changes in proteins of the cells
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.381.pdf
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