ISSN:
1420-9071
Keywords:
L-arginine
;
L-arginine transport
;
L-arginine uptake
;
rat brain
;
synaptosomes
;
nerve endings
;
amino acid uptake
;
transport systems
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The uptake ofL-arginine into purified rat brain synaptosomes was investigated with respect to time and various concentrations ofL-[3H] arginine. Specific uptake was found to be linear with time for up to 5 min of incubation at 37°C. Electrolytes, including sodium chloride, potassium chloride, magnesium chloride and calcium chloride, inhibited uptake of 3 μML-arginine, and the inhibitory effect increased with increased electrolyte concentration under constant osmolarity. It was found thatL-arginine was transported into synaptosomes by two uptake components — a high affinity component (3.5 μM) and a low affinity component (100 μM). These two components were similar to the Ly+ system because of their extreme sensitivity to inhibition byL-lysine andL-ornithine but were distinguishable from each other by kinetic analysis of the uptake data and by their relative sensitivity to inhibition by several amino acids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01946914
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