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  • 1
    ISSN: 1432-0878
    Keywords: Key words: Endoderm ; Sensory cells ; Hydra magnipapillata (Cnidaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The free surface of the endoderm of Hydra magnipapillata was observed by scanning electron microscopy in order to analyze the fine structure of the apical receptive sites of the sensory cells. Endodermal sensory cells had a small round exposed surface provided with a single cilium. Two types of sensory cells, tufted and papillary, could be distinguished from each other by their apical features. The former projected 50 or more microvilli on their apical surface, which was at level with the surface of neighboring epithelial cells. The latter displayed a rounded swelling on their apex, on which 10–30 microvilli of various lengths were found pointing random directions. Both types of receptor regions were compared with those of previously reported sensory cells in Hydra ectoderm and in the gut epithelium of other animal species.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Intestine, small ; Intraepithelial lymphocytes ; Microcinematography ; Ultrastructure ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In previous ultrastructural studies we have shown that at the tip of intestinal villi in guinea pigs, effete enterocytes are separated into two portions: a thin apical cytoplasm to be exfoliated into the lumen and a major basal portion to be ingested by lamina propria macrophages. During this process, intraepithelially disposed, large granular lymphocytes interdigitate with enterocytes in a complex manner. In the present study, the relation between the enterocytes and the lymphocytes in the villous epithelium of the guinea pig small intestine is described by use of transmission and scanning electron microscopy in an attempt to visualize the roles and activities of the lymphocytes more clearly. The lymphocytes project numerous pointed processes into effete enterocytes, even piercing them. Enterocytes are deeply indented or perforated, probably as a result of the encroaching lymphocyte processes. Some enterocytes are separated into apical and basal portions by numerous large excavations in the cytoplasm. These findings indicate that repeated perforating penetration of the lymphocytes induces cell cleavage. Supporting this supposition, our microcinematographic observations demonstrate the alternate protrusion and withdrawal of processes of lymphocytes. The processes advance with a pointed end, and subsequently, retract with a rounded end in a cycle of 8–18 seconds.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 264 (1991), S. 269-281 
    ISSN: 1432-0878
    Keywords: Interstitial tissue ; Kidney ; Scanning electron microscopy ; Vasa recta ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cytoarchitecture of the interstitial tissue of the rat kidney was studied by combined scanning and transmission electron microscopy. The renal interstitium is composed of an elaborate network of stellate sustentacular cells. In the cortex, sustentacular cells radiate thin branching processes to form a fine reticulum, which supports intertubular spaces. In the medulla, these cells extend thick processes horizontally along the basal surfaces of the thin limbs or vasa recta, reinforcing their attenuate walls. The horizontal processes connect with each other at their terminals, compartmentalizing the interstitial space into thin layers. The medullary sustentacular cells contain abundant small lipid droplets. The network of sustentacular cells houses vasa recta, keeping them in parallel position to each other and to the tubules. The arterial vasa recta are accompanied by pericytes, which frequently contain lipid droplets larger in size than those in the sustentacular cells. Venous vasa recta extend numerous basal microvilli, which anchor the venous wall to adjacent tubules or vessels. Numerous free cells, round in shape, are found in the sustentacular cell network, especially in the cortex. They consist of macrophages and occasional lymphocytes. Some macrophages extend long pseudopodia, while others make intimate contact with lymphocytes, suggesting their high level of activity.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0878
    Keywords: Key words: Intestine ; small ; Intraepithelial lymphocytes ; Microcinematography ; Ultrastructure ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In previous ultrastructural studies we have shown that at the tip of intestinal villi in guinea pigs, effete enterocytes are separated into two portions: a thin apical cytoplasm to be exfoliated into the lumen and a major basal portion to be ingested by lamina propria macrophages. During this process, intraepithelially disposed, large granular lymphocytes interdigitate with enterocytes in a complex manner. In the present study, the relation between the enterocytes and the lymphocytes in the villous epithelium of the guinea pig small intestine is described by use of transmission and scanning electron microscopy in an attempt to visualize the roles and activities of the lymphocytes more clearly. The lymphocytes project numerous pointed processes into effete enterocytes, even piercing them. Enterocytes are deeply indented or perforated, probably as a result of the encroaching lymphocyte processes. Some enterocytes are separated into apical and basal portions by numerous large excavations in the cytoplasm. These findings indicate that repeated perforating penetration of the lymphocytes induces cell cleavage. Supporting this supposition, our microcinematographic observations demonstrate the alternate protrusion and withdrawal of processes of lymphocytes. The processes advance with a pointed end, and subsequently, retract with a rounded end in a cycle of 8–18 seconds.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 277 (1994), S. 79-86 
    ISSN: 1432-0878
    Keywords: Myoepithelial cells ; Nerve cells ; Sensory cells ; Hydra magnipapillata (Cnidaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The fine structure of the ectodermal and endodermal muscle layers of Hydra magnipapillata has been analyzed by scanning electron microscopy after hydrolytic removal of the mesoglea with NaOH and subsequent exposure of the basal and lateral aspects of the layers by mechanical dissection. The ectodermal muscle layer consists of fibrous processes of epithelial cells extending longitudinally to the body axis, whereas the endodermal muscle layer comprises cells with hexagonal bases and several strands of myonemes oriented circularly. In each layer, the muscular elements tightly interdigitate, extending a continuous muscle sheet along the mesoglea. The ectodermal and endodermal muscle sheets communicate with each other via foliate microprojections penetrating the mesoglea. On the lateral aspect of the ectodermal epithelium, spiny nerve fibers run along the upper surface of the muscle processes. The spines are often attached to muscle processes, suggesting that the former monitor muscle contraction. Nerve fibers occasionally come into contact with the mesoglea through narrow gaps between the muscle processes. In the hypostomal ectoderm, a small spindle-shaped cell, probably sensory in nature, extends an apical cilium and a long basal process.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 277 (1994), S. 79-86 
    ISSN: 1432-0878
    Keywords: Key words: Myoepithelial cells – Nerve cells – Sensory cells –Hydra magnipapillata (Cnidaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The fine structure of the ectodermal and endodermal muscle layers of Hydra magnipapillata has been analyzed by scanning electron microscopy after hydrolytic removal of the mesoglea with NaOH and subsequent exposure of the basal and lateral aspects of the layers by mechanical dissection. The ectodermal muscle layer consists of fibrous processes of epithelial cells extending longitudinally to the body axis, whereas the endodermal muscle layer comprises cells with hexagonal bases and several strands of myonemes oriented circularly. In each layer, the muscular elements tightly interdigitate, extending a continuous muscle sheet along the mesoglea. The ectodermal and endodermal muscle sheets communicate with each other via foliate microprojections penetrating the mesoglea. On the lateral aspect of the ectodermal epithelium, spiny nerve fibers run along the upper surface of the muscle processes. The spines are often attached to muscle processes, suggesting that the former monitor muscle contraction. Nerve fibers occasionally come into contact with the mesoglea through narrow gaps between the muscle processes. In the hypo-stomal ectoderm, a small spindle-shaped cell, probably sensory in nature, extends an apical cilium and a long basal process.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 242 (1985), S. 57-66 
    ISSN: 1432-0878
    Keywords: Lamina propria ; Intestinal mucosa ; Reticular tissue ; Scanning electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The three-dimensional architecture of the lamina propria in the jejunal mucosa of the rat was studied by scanning electron microscopy. The lamina propria is a typical reticular tissue with fine nets of reticular fibers and free and fixed cells lying among them. However, the lamina propria in the core of villi displayed structural features closer to other reticular or lymphoid tissues than that among the crypts. In the villi, the tissue was supported by a spongy framework of interconnecting fibroblasts, and densely infiltrated by free cells. Among the crypts, cellular elements were rather sparsely dispersed in a complex network of reticular fibers. A thin layer of reticular fibers lined the basal lamina of the epithelium. This layer contained a network of flattened cells which anastomosed with each other via slender processes. The basal lamina and the fibrous layer were perforated with round pores, through which free cells or basal processes of the epithelial cells passed. Many macrophages were found resting on the reticular framework of the lamina propria, frequently in close association with immunoblast-like cells. This paper further includes scanning electron-microscopic observations on the central lacteal with special reference to its luminal projections and trabeculae.
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  • 8
  • 9
    Publication Date: 1994-07-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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  • 10
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