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  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of reduction of metmyoglobins by ascorbate. Effect of the modification of the heme distal side, heme propionates, and 2,4-substituents of deuterohemin (1986)
    s.l. : American Chemical Society
    Inorganic chemistry 25 (1986), S. 4756-4760 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00246a034
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  • 2
    Electronic Resource
    Electronic Resource
    Separation and Purification of Organic Acids by Gas Antisolvent Crystallization (1994)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 42 (1994), S. 1993-1997 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00045a030
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  • 3
    Electronic Resource
    Electronic Resource
    Generation and dissociation of complexes of iron and phosphorus atoms in silicon (1995)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 78 (1995), S. 3077-3082 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: To examine the possibility of generating complexes of iron and phosphorus atoms in silicon, neutral interstitial iron in phosphorus doped silicon crystals with various concentrations were measured with the electron spin resonance (ESR) method after cooling from high temperature at various cooling rates. This concentration was found to depend on the cooling rate when the concentration of phosphorus was high: The concentration of interstitial iron was low only when the concentration of phosphorus was high and the cooling rate was slow. This result shows that some iron atoms form complexes with phosphorus atoms during cooling and become ESR-inactive. Annealing behaviors of these complexes monitored as to the concentration of neutral interstitial iron are described as a combination of two processes, namely, the release of iron atoms from iron-phosphorus complexes and the diffusion of iron to quenched-in defects which are concluded to exist based on the dependence of annealing behavior on the cooling rate. From the analysis of annealing behaviors, the binding energy between iron and phosphorus atoms and the activation energy of diffusion of iron were determined to be 0.90 and 0.80 eV, respectively. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.360059
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  • 4
    Electronic Resource
    Electronic Resource
    MACRONUTRIENT UTILIZATION BY PHOTOSYNTHETIC EUKARYOTES AND THE FABRIC OF INTERACTIONS (2001)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology and Plant Molecular Biology 52 (2001), S. 163-210 
    Details
    ISSN: 1040-2519
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Abstract Organisms acclimate to a continually fluctuating nutrient environment. Acclimation involves responses specific for the limiting nutrient as well as responses that are more general and occur when an organism experiences different stress conditions. Specific responses enable organisms to efficiently scavenge the limiting nutrient and may involve the induction of high-affinity transport systems and the synthesis of hydrolytic enzymes that facilitate the release of the nutrient from extracellular organic molecules or from internal reserves. General responses include changes in cell division rates and global alterations in metabolic activities. In photosynthetic organisms there must be precise regulation of photosynthetic activity since when severe nutrient limitation prevents continued cell growth, excitation of photosynthetic pigments could result in the formation of reactive oxygen species, which can severely damage structural and functional features of the cell. This review focuses on ways that photosynthetic eukaryotes assimilate the macronutrients nitrogen, sulfur, and phosphorus, and the mechanisms that govern assimilatory activities. Also discussed are molecular responses to macronutrient limitation and the elicitation of those responses through integration of environmental and cellular cues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.arplant.52.1.163
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  • 5
    Electronic Resource
    Electronic Resource
    The function of SULTR2;1 sulfate transporter during seed development in Arabidopsis thaliana (2005)
    Oxford, UK; Malden, USA : Munksgaard International Publishers
    Physiologia plantarum 125 (2005), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SULTR2;1 is a low-affinity sulfate transporter expressed in the vascular tissues of roots and leaves for interorgan transport of sulfate in Arabidopsis thaliana. Transgenic Arabidopsis carrying a fusion gene construct of SULTR2;1 5′-promoter region and β-glucuronidase coding sequence (GUS) demonstrated that within the reproductive tissues, SULTR2;1 is specifically expressed in the bases and veins of siliques and in the funiculus, which connects the seeds and the silique. The antisense suppression of SULTR2;1 mRNA caused decrease of sulfate contents in seeds and of thiol contents both in seeds and leaves, as compared with the wildtype (WT). The effect of antisense suppression of SULTR2;1 on seed sulfur status was determined by introducing a sulfur-indicator construct, p35S::βSRx3:GUS, which drives the expression of GUS reporter under a chimeric cauliflower mosaic virus 35S promoter containing a triplicate repeat of sulfur-responsive promoter region of soybean β-conglycinin β subunit (βSRx3). The mature seeds of F1 plants carrying both the SULTR2;1 antisense and p35S::βSRx3:GUS constructs exhibited significant accumulation of GUS activities on sulfur deficiency, as compared with those carrying only the p35S::βSRx3:GUS construct in the WT background. These results suggested that SULTR2;1 is involved in controlling translocation of sulfate into developing siliques and may modulate the sulfur status of seeds in A. thaliana.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.2005.00543.x
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  • 6
    Electronic Resource
    Electronic Resource
    A comparative study of floral development inTrillium apetalon andT. kamtschaticum (Liliaceae) (1994)
    Springer
    Journal of plant research 107 (1994), S. 237-243 
    Details
    ISSN: 1618-0860
    Keywords: Development ; Flower ; Liliaceae ; Serial homeosis ; Trillium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Trillium apetalon Makino is unique amongTrillium in having apetalous flowers. Using scanning electron microscope, the early floral development was observed in comparison with that ofT. kamtschaticum Pallas ex Pursh having petalous flowers. Morphologically petal primordia closely resemble stamen primordia in their more or less narrow and radially symmetric shape and are clearly distinct from sepal primordia with broad bases. Early in floral development sepal primordia are first initiated and subsequently two whorls of three primordia each are formed in rapid sequence, the first three at the corners and the second three at the sides of the triangular floral apex. Based on comparison in position and early developmental processes of their primordia, petals and outer stamens ofTrillium kamtschaticum are equivalent to outer stamens and inner stamens ofT. apetalon. The replacement of petals by outer stamens apparently leads to the loss of petals inTrillium apetalon flowers. Such a replacement can be interpreted in terms of homeosis. The replacement of the petal whorl leads to the serial replacement of the subsequent whorls: outer stamens by inner stamens, and inner stamens by gynoecium inTrillium apetalon. The term ‘serial homeosis’ is introduced for this serial replacement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344250
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  • 7
    Electronic Resource
    Electronic Resource
    A protein in the oxygen-evolving complex in the chloroplast is associated with symptom expression on tobacco leaves infected with cucumber mosaic virus strain Y (1991)
    Springer
    Plant molecular biology 16 (1991), S. 689-698 
    Details
    ISSN: 1573-5028
    Keywords: cucumber mosaic virus ; differential expression ; oxygen-evolving complex protein ; symptom expression ; tobacco ; virus infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To elucidate the molecular basis of symptom expression in virus-infected plants, the changes in proteins between tobacco, Nicotiana tabacum cv. Ky57, leaves inoculated with cucumber mosaic virus strain Y [CMV(Y)] and strain O [CMV(O)], were compared by 2-dimensional (2-D) gel electrophoresis. The appearance of chlorotic spots in CMV(Y)-inoculated tobacco leaves accompanied an increase of 3 polypeptides and a decrease in 6 polypeptides, as compared with those in the CMV(O)-inoculated tobacco which showed no clear symptoms. The decrease in the amounts of two polypeptides of 22 and 23 kDa was particularly significant: these two polypeptides were compared with a 24 kDa polypeptide, which co-migrated with them in 2-D gel electrophoresis but did not clearly decrease at an early stage of infection, as well as major other proteins of CMV(Y)-inoculated tobacco leaves. However, the 22, 23 and 24 kDa polypeptides showed the same peptide mapping pattern. Furthermore, the 12 amino acid residues at N-termini of the three polypeptides match those of the extrinsic 23 kDa polypeptide of an oxygen-evolving complex from spinach. A comparative analysis of the 22, 23 and 24 kDa polypeptides in N. tabacum and its ancestral parents, N. sylvestris and N. tomentosiformis, revealed that the 22 kDa polypeptide derives from N. sylvestris and the 23 kDa polypeptide from N. tomentosiformis; the 24 kDa polypeptide derives from both ancestral Nicotiana species. The results indicate that the polypeptides whose amounts differentially decrease with the progress of symptom expression in N. tabacum inoculated with CMV(Y) are one component of the oxygen-evolving complex in photosystem II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023433
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular cloning, characterization and expression of cDNA encoding phosphoserine aminotransferase involved in phosphorylated pathway of serine biosynthesis from spinach (1997)
    Springer
    Plant molecular biology 33 (1997), S. 359-366 
    Details
    ISSN: 1573-5028
    Keywords: cDNA cloning ; phosphoserine aminotransferase ; serine biosynthesis ; spinach ; Spinacia oleracea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphoserine aminotransferase (PSA) catalyzes the conversion of phosphohydroxypyruvate to phosphoserine in the phosphorylated pathway of serine biosynthesis. A cDNA clone encoding PSA was isolated from the cDNA library of spinach (Spinacia oleracea L.) green leaves. Determination of the nucleotide sequence revealed the presence of an open reading frame encoding 430 amino acids, exhibiting 38-50% homology with the amino acid sequences of bacterial, yeast and animal PSA. It contains an N-terminal extension of ca. 60 amino acids in addition to the sequences from other organisms. The general features of plastidic transit peptide are observed in this N-terminal sequence, suggesting the plastid localization of the PSA protein encoded by this cDNA. The bacterial expression of the cDNA could functionally rescue the auxotrophy of serine in the serC- mutant, Escherichia coli KL282. The enzymatic activity of PSA was demonstrated in vitro in the extracts of E. coli over-expressing the cDNA. Southern blot analysis indicated the presence of a couple of related genes (Psa) in the spinach genome. RNA blot hybridization suggested the preferential expression of the Psa gene in the roots of green seedlings and in the suspension cells cultured under a dark condition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005730725764
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  • 9
    Electronic Resource
    Electronic Resource
    Calcium chloride as a major component contributing to aphid resistance ofNicotiana benthamiana (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 1579-1589 
    Details
    ISSN: 1573-1561
    Keywords: Nicotiana benthamiana ; Myzus persicae ; green peach aphid ; Nicotiana gossei ; calcium chloride ; plant resistance to insects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Substances with antiaphid activity were extracted from the leaf surface of aphid-resistantN. benthamiana and purified. Sugar esters and diacyl glycerols were isolated from the chloroform extract, but only diacyl glycerols showed significant activity. However, the major activity was found in the water extract rather than in the chloroform extract. From the water extract calcium chloride was isolated as the most abundant active substance. Only calcium chloride showed significant activity among several calcium salts and chlorides of several metals that are abundant in plants. Calcium contents per unit area of leaf surface ofN. benthamiana and aphid-resistantN. gossei were almost equal to each other and 10–100 times higher than that of aphid-susceptibleN. tabacum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02272399
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation of Molecular Markers Linked to the Cry Locus Conferring Resistance to Cucumber mosaic cucumovirus Infection in Cowpea (2000)
    Springer
    Journal of general plant pathology 66 (2000), S. 242-250 
    Details
    ISSN: 1610-739X
    Keywords: Key words : CMV, cowpea, RAPD, RFLP, resistance gene analog, linkage analysis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: We previously demonstrated that cowpea [Vigna unguiculata (L.) Walp.] cultivar Kurodane-Sanjaku contains the Cry gene, which confers resistance against Cucumber mosaic cucumovirus infection. In this paper, randomly amplified polymorphic DNA (RAPD) analysis was carried out to tag the Cry locus. Bulked segregant analysis for RAPD resulted in many polymorphisms in amplified DNA patterns. Candidates were further screened using parental and/or F2 cowpea DNAs. As a result, we obtained three RAPD markers, D13/E14-350, WA3-850 and OPE3-500, flanking the Cry locus. In addition, we amplified cowpea sequences coding for the putative nucleotide-binding site (NBS). Degenerate primers based on NBS sequences of tobacco N and Arabidopsis RPS2 disease resistance genes were used for polymerase chain reaction, and resultant products were cloned and sequenced. Among eight independent clones, cowpea resistance gene analog (CRGA) 5 showed a distinct polymorphism when used as a probe for restriction fragment length polymorphism analysis against the susceptible cowpea cultivar PI 189375 and a near-isogenic line for the Cry. Linkage analyses of these molecular markers showed that genetic distances of CRGA5, D13/E14-350, WA3-850 and OPE3-500 to the Cry locus were 0.7, 5.2, 11.5 and 24.5 cM, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012953
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