Publication Date:
2014-01-31
Description:
We recently discovered an unexpected phenomenon of somatic cell reprogramming into pluripotent cells by exposure to sublethal stimuli, which we call stimulus-triggered acquisition of pluripotency (STAP). This reprogramming does not require nuclear transfer or genetic manipulation. Here we report that reprogrammed STAP cells, unlike embryonic stem (ES) cells, can contribute to both embryonic and placental tissues, as seen in a blastocyst injection assay. Mouse STAP cells lose the ability to contribute to the placenta as well as trophoblast marker expression on converting into ES-like stem cells by treatment with adrenocorticotropic hormone (ACTH) and leukaemia inhibitory factor (LIF). In contrast, when cultured with Fgf4, STAP cells give rise to proliferative stem cells with enhanced trophoblastic characteristics. Notably, unlike conventional trophoblast stem cells, the Fgf4-induced stem cells from STAP cells contribute to both embryonic and placental tissues in vivo and transform into ES-like cells when cultured with LIF-containing medium. Taken together, the developmental potential of STAP cells, shown by chimaera formation and in vitro cell conversion, indicates that they represent a unique state of pluripotency.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Obokata, Haruko -- Sasai, Yoshiki -- Niwa, Hitoshi -- Kadota, Mitsutaka -- Andrabi, Munazah -- Takata, Nozomu -- Tokoro, Mikiko -- Terashita, Yukari -- Yonemura, Shigenobu -- Vacanti, Charles A -- Wakayama, Teruhiko -- England -- Nature. 2014 Jan 30;505(7485):676-80. doi: 10.1038/nature12969.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉1] Laboratory for Cellular Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan [2] Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan [3] Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. ; Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan. ; Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan. ; Genome Resource and Analysis Unit, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan. ; Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan. ; 1] Laboratory for Cellular Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan [2] Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan. ; Electron Microscopy Laboratory, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan. ; Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. ; 1] Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan [2] Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24476891" target="_blank"〉PubMed〈/a〉
Keywords:
Adrenocorticotropic Hormone/pharmacology
;
Animals
;
*Cell Differentiation/drug effects/genetics
;
Cell Lineage/drug effects
;
*Cellular Reprogramming/drug effects
;
Embryonic Stem Cells/*cytology/drug effects/metabolism
;
Epigenesis, Genetic/drug effects/genetics
;
Female
;
Fibroblast Growth Factor 4/pharmacology
;
Induced Pluripotent Stem Cells/*cytology/drug effects
;
Leukemia Inhibitory Factor/pharmacology
;
Mice
;
Mice, Inbred ICR
;
Placenta/*cytology/drug effects
;
Pregnancy
;
Trophoblasts/*cytology/drug effects
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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