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    Materials, Vol. 11, Pages 2574: Fabrication of Silver Nanoparticles Using a Gas Phase Nanocluster Device and Preliminary Biological Uses (2018)
    MDPI
    In: Materials
    Details
    Publication Date: 2018
    Description: Nanoparticles can be used in a large variety of applications, including magnetic sensing, biological, superconductivity, tissue engineering, and other fields. In this study, we explore the fabrication of gas phase silver nanoparticles using a sputtering evaporation source. This setup composed of a dual magnetron cluster source holds several advantages over other techniques. The system has independent control over the cluster concentration and a wide range of cluster size and materials that can be used for the clusters and for the matrix where it can be embedded. Characterization of these silver nanoparticles was done using transmission electron microscopy (TEM). We obtain a lateral width of 10.6 nm with a dispersion of 0.24 nm. With atomic force microscopy (AFM) a Gaussian fit of this distribution yields and average height of 6.3 nm with a standard deviation of 1.4 nm. We confirm that the deposited silver nanoparticles have a homogenous area distribution, that they have a defined shape and size distribution, and that they are single standing nanoparticles. Given that the scientific literature is not precise regarding the toxic concentration of the nanoparticles, devices such as ours can help clarify these questions. In order to explore further biological applications, we have done preliminary experiments of cell spreading (myoblast adhesion), obtaining interesting morphological changes correlated with the silver concentration on the surface. With a deposited silver concentration ranging from 100–620 ng/cm2, the cells showed morphological changes in a short time of 2 h. We conclude that this high precision nanoparticle fabrication technique is adequate for further biological research.
    Electronic ISSN: 1996-1944
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by MDPI
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  • 2
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    Flexible minerals: self-assembled calcite spicules with extreme bending strength (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2013-03-16
    Description: Silicatein-alpha is responsible for the biomineralization of silicates in sponges. We used silicatein-alpha to guide the self-assembly of calcite "spicules" similar to the spicules of the calcareous sponge Sycon sp. The self-assembled spicules, 10 to 300 micrometers (mum) in length and 5 to 10 mum in diameter, are composed of aligned calcite nanocrystals. The spicules are initially amorphous but transform into calcite within months, exhibiting unusual growth along [100]. They scatter x-rays like twinned calcite crystals. Whereas natural spicules evidence brittle failure, the synthetic spicules show an elastic response, which greatly enhances bending strength. This remarkable feature is linked to a high protein content. With nano-thermogravimetric analysis, we measured the organic content of a single spicule to be 10 to 16%. In addition, the spicules exhibit waveguiding properties even when they are bent.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Natalio, Filipe -- Corrales, Tomas P -- Panthofer, Martin -- Schollmeyer, Dieter -- Lieberwirth, Ingo -- Muller, Werner E G -- Kappl, Michael -- Butt, Hans-Jurgen -- Tremel, Wolfgang -- New York, N.Y. -- Science. 2013 Mar 15;339(6125):1298-302. doi: 10.1126/science.1216260.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut fur Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universitat, Mainz, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23493708" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Calcium Carbonate/*chemistry ; Cathepsins/*chemistry ; Microscopy, Electron, Transmission ; Nanoparticles/chemistry ; Porifera ; Spectroscopy, Fourier Transform Infrared ; *Stress, Mechanical
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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