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  • 1
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    Functional implication of archaeal homologues of human RNase P protein pair Pop5 and Rpp30 (2016)
    Oxford University Press
    Details
    Publication Date: 2016-01-09
    Description: Pho Pop5 and Pho Rpp30 in the hyperthermophilic archaeon Pyrococcus horikoshii , homologues of human ribonuclease P (RNase P) proteins hPop5 and Rpp30, respectively, fold into a heterotetramer [ Pho Rpp30–( Pho Pop5) 2 – Pho Rpp30], which plays a crucial role in the activation of RNase P RNA ( Pho pRNA). Here, we examined the functional implication of Pho Pop5 and Pho Rpp30 in the tetramer. Surface plasmon resonance (SPR) analysis revealed that the tetramer strongly interacts with an oligonucleotide including the nucleotide sequence of a stem-loop SL3 in Pho pRNA. In contrast, Pho Pop5 had markedly reduced affinity to SL3, whereas Pho Rpp30 had little affinity to SL3. SPR studies of Pho Pop5 mutants further revealed that the C-terminal helix (α4) in Pho Pop5 functions as a molecular recognition element for SL3. Moreover, gel filtration indicated that Pho Rpp30 exists as a monomer, whereas Pho Pop5 is an oligomer in solution, suggesting that Pho Rpp30 assists Pho Pop5 in attaining a functionally active conformation by shielding hydrophobic surfaces of Pho Pop5. These results, together with available data, allow us to generate a structural and mechanistic model for the Pho pRNA activation by Pho Pop5 and Pho Rpp30, in which the two C-terminal helices (α4) of Pho Pop5 in the tetramer whose formation is assisted by Pho Rpp30 act as binding elements and bridge SL3 and SL16 in Pho pRNA.
    Print ISSN: 0021-924X
    Electronic ISSN: 1756-2651
    Topics: Biology , Chemistry and Pharmacology
    Published by Oxford University Press on behalf of The Japanese Biochemical Society (JBS).
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  • 2
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    Stable complex formation between serine protease inhibitor and zymogen: coagulation factor X cleaves the Arg393-Ser394 bond in a reactive centre loop of antithrombin in the presence of heparin (2012)
    Oxford University Press
    Details
    Publication Date: 2012-10-30
    Description: Antithrombin (AT) inhibits several blood coagulation proteases, including activated factor X (FXa), by forming stable complexes with these proteases. Herein, we demonstrate that AT forms a stable complex with zymogen factor X (FX). Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) and size-exclusion chromatography analyses showed that AT and FX formed an SDS-stable complex, which is distinct in apparent molecular mass from an FXa–AT complex, in the presence of heparin. Amino-terminal sequence analysis of the complex following SDS–PAGE under reducing conditions provided clear evidence that AT forms this complex with the heavy chain of FX, because two sequences, HGSPVDI (residues 1–7 of AT) and SVAQATS (residues 1–7 of the heavy chain of FX), were identified. Furthermore, sequence SLNPNRV, which corresponds to residues 394–400 of AT, was identified in the non-reduced FX–AT complex, indicating that FX cleaved the Arg393–Ser394 bond in a reactive centre loop of AT. Unfractionated heparin induced FX–AT complex formation more effectively than low-molecular weight heparin or AT-binding pentasaccharide, and appeared to promote complex formation mainly via a template effect. These data suggest that AT is capable of forming a stable complex with zymogen FX by acting as an inhibitor in the presence of heparin.
    Print ISSN: 0021-924X
    Electronic ISSN: 1756-2651
    Topics: Biology , Chemistry and Pharmacology
    Published by Oxford University Press on behalf of The Japanese Biochemical Society (JBS).
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  • 3
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    Recent Progress in High Performance Ag-Sheathed Bi2223 Wire (DI-BSCCO ® ) (2011)
    Institute of Physics (IOP)
    Details
    Publication Date: 2011-07-23
    Description: Sumitomo Electric has been developing the silver-sheathed Bi2223 multi-filamentary wires since the discovery of Bi-based superconductors. DI-BSCCO is the high performance wires produced using the controlled-overpressure (CT-OP) sintering technique. The present commercial DI-BSCCO can provide the uniform high critical current, Ic , up to 180 A with length over 2000 m, and recently 200 A were succeeded to be obtained by the same kind of 1000 m length wires, resulting from the improvement and control of the microstructure in Bi2223 multi-filaments. The short trial wires of several meters have exhibited the highest Ic over 240 A at 77K in self-field (corresponding to 580 A per 1 cm-width). Besides, the optimization of carrier density after CT-OP led to further enhancement of Ic , reached 250 A. All the derivative products also have uniform critical current properties over entire length even after lamination with the reinforcing metals. The performances of DI-BSCCO...
    Print ISSN: 1757-8981
    Electronic ISSN: 1757-899X
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Institute of Physics (IOP)
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  • 4
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    Study on flow instability and countermeasure in a draft tube with swirling flow (2014)
    Institute of Physics (IOP)
    Details
    Publication Date: 2014-12-11
    Description: The swirling flow in the draft tube of a Francis turbine can cause the flow instability and the cavitation surge and has a larger influence on hydraulic power operating system. In this paper, the cavitating flow with swirling flow in the diffuser was studied by the draft tube component experiment, the model Francis turbine experiment and the numerical simulation. In the component experiment, several types of fluctuations were observed, including the cavitation surge and the vortex rope behaviour by the swirling flow. While the cavitation surge and the vortex rope behaviour were suppressed by the aeration into the diffuser, the loss coefficient in the diffuser increased by the aeration. In the model turbine test the aeration decreased the efficiency of the model turbine by several percent. In the numerical simulation, the cavitating flow was studied using Scale-Adaptive Simulation (SAS) with particular emphasis on understanding the unsteady characteristics of the vortex rope stru...
    Print ISSN: 1755-1307
    Electronic ISSN: 1755-1315
    Topics: Geography , Geosciences , Physics
    Published by Institute of Physics (IOP)
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  • 5
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    Phytochrome controls alternative splicing [Plant Biology] (2014)
    National Academy of Sciences
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    Publication Date: 2014-12-31
    Description: Plants monitor the ambient light conditions using several informational photoreceptors, including red/far-red light absorbing phytochrome. Phytochrome is widely believed to regulate the transcription of light-responsive genes by modulating the activity of several transcription factors. Here we provide evidence that phytochrome significantly changes alternative splicing (AS) profiles at the genomic level...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 6
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    Spatial coupling of mTOR and autophagy augments secretory phenotypes (2011)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2011-04-23
    Description: Protein synthesis and autophagic degradation are regulated in an opposite manner by mammalian target of rapamycin (mTOR), whereas under certain conditions it would be beneficial if they occurred in unison to handle rapid protein turnover. We observed a distinct cellular compartment at the trans side of the Golgi apparatus, the TOR-autophagy spatial coupling compartment (TASCC), where (auto)lysosomes and mTOR accumulated during Ras-induced senescence. mTOR recruitment to the TASCC was amino acid- and Rag guanosine triphosphatase-dependent, and disruption of mTOR localization to the TASCC suppressed interleukin-6/8 synthesis. TASCC formation was observed during macrophage differentiation and in glomerular podocytes; both displayed increased protein secretion. The spatial coupling of cells' catabolic and anabolic machinery could augment their respective functions and facilitate the mass synthesis of secretory proteins.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426290/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426290/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Narita, Masako -- Young, Andrew R J -- Arakawa, Satoko -- Samarajiwa, Shamith A -- Nakashima, Takayuki -- Yoshida, Sei -- Hong, Sungki -- Berry, Lorraine S -- Reichelt, Stefanie -- Ferreira, Manuela -- Tavare, Simon -- Inoki, Ken -- Shimizu, Shigeomi -- Narita, Masashi -- DK083491/DK/NIDDK NIH HHS/ -- R01 DK083491/DK/NIDDK NIH HHS/ -- R01 DK083491-03/DK/NIDDK NIH HHS/ -- Cancer Research UK/United Kingdom -- New York, N.Y. -- Science. 2011 May 20;332(6032):966-70. doi: 10.1126/science.1205407. Epub 2011 Apr 21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cancer Research UK Cambridge Research Institute (CRI), Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21512002" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acids/metabolism ; Animals ; *Autophagy ; *Cell Aging ; Cell Line ; Cytoplasm/metabolism ; Cytoplasmic Vesicles/*metabolism/ultrastructure ; Endoplasmic Reticulum, Rough/ultrastructure ; Genes, ras ; Golgi Apparatus/ultrastructure ; HL-60 Cells ; Humans ; Interleukin-6/metabolism ; Interleukin-8/metabolism ; Lysosomes/metabolism/ultrastructure ; Mice ; Monomeric GTP-Binding Proteins/genetics/metabolism ; Nocodazole/pharmacology ; Phagosomes/metabolism/ultrastructure ; Phenotype ; Podocytes/metabolism/ultrastructure ; Protein Biosynthesis ; Proteins/*secretion ; TOR Serine-Threonine Kinases/*metabolism ; Vacuoles/ultrastructure ; trans-Golgi Network/metabolism/ultrastructure
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 7
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    Osteoprotection by semaphorin 3A (2012)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2012-04-24
    Description: The bony skeleton is maintained by local factors that regulate bone-forming osteoblasts and bone-resorbing osteoclasts, in addition to hormonal activity. Osteoprotegerin protects bone by inhibiting osteoclastic bone resorption, but no factor has yet been identified as a local determinant of bone mass that regulates both osteoclasts and osteoblasts. Here we show that semaphorin 3A (Sema3A) exerts an osteoprotective effect by both suppressing osteoclastic bone resorption and increasing osteoblastic bone formation. The binding of Sema3A to neuropilin-1 (Nrp1) inhibited receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast differentiation by inhibiting the immunoreceptor tyrosine-based activation motif (ITAM) and RhoA signalling pathways. In addition, Sema3A and Nrp1 binding stimulated osteoblast and inhibited adipocyte differentiation through the canonical Wnt/beta-catenin signalling pathway. The osteopenic phenotype in Sema3a-/- mice was recapitulated by mice in which the Sema3A-binding site of Nrp1 had been genetically disrupted. Intravenous Sema3A administration in mice increased bone volume and expedited bone regeneration. Thus, Sema3A is a promising new therapeutic agent in bone and joint diseases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hayashi, Mikihito -- Nakashima, Tomoki -- Taniguchi, Masahiko -- Kodama, Tatsuhiko -- Kumanogoh, Atsushi -- Takayanagi, Hiroshi -- England -- Nature. 2012 May 3;485(7396):69-74. doi: 10.1038/nature11000.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Signaling, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-ku, Tokyo 113-8549, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22522930" target="_blank"〉PubMed〈/a〉
    Keywords: Adipocytes/cytology/drug effects ; Animals ; Bone Marrow Cells/cytology/metabolism ; *Bone Resorption/drug therapy ; Cell Differentiation/drug effects ; Cells, Cultured ; Coculture Techniques ; *Cytoprotection/drug effects ; Female ; Male ; Mice ; Neuropilin-1/metabolism ; Osteoblasts/*cytology/drug effects ; Osteoclasts/*cytology/drug effects ; *Osteogenesis/drug effects ; Semaphorin-3A/deficiency/genetics/*metabolism/pharmacology ; Skull/cytology ; Wnt Signaling Pathway/drug effects
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
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  • 8
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    Study on flow instability in a diffuser with swirling flow under several conditions of pipe length and swirl intensity (2016)
    Institute of Physics (IOP)
    Details
    Publication Date: 2016-12-13
    Description: A swirling flow in a diffuser such as a draft tube of a hydro turbine may induce the flow instabilities accompanied by pressure fluctuations known as vortex rope behaviour and cavitation surge. Cavitation surge is the self-excited oscillation, which induces the large flow rate fluctuation that results from the change of the cavity volume. In this research, the investigation of the effect of the pipe length and the swirl intensity on the flow instabilities in a diffuser was performed by experiments and numerical analyses using the draft tube component experimental facility. The length of the pipe was modified by up to about 25 times as long as the diameter of the throat in order to validate the one-dimensional analyses. In addition, the swirl intensity was changed by replacing another swirl generator. The frequency of cavitation surge was changed with regard to the swirl intensity as the one-dimensional analyses in the previous study has predicted it. Unsteady numerical simulatio...
    Print ISSN: 1755-1307
    Electronic ISSN: 1755-1315
    Topics: Geography , Geosciences , Physics
    Published by Institute of Physics (IOP)
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  • 9
    Electronic Resource
    Electronic Resource
    Symmetries of hydrogen-bonded enol forms of diketones as determined by x-ray photoelectron spectroscopy (1979)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 101 (1979), S. 3157-3162 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00506a003
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Mechanical Properties of Composite Resin for Bone Cement and Stress Analysis of Bone Cement in THR (1997)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 137 (Apr. 1997), p. 203-206 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/44/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.137.203.pdf
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