Publication Date:
1993-07-16
Description:
Fertilization is accompanied by a transient increase in the concentration of intracellular Ca2+, which serves as a signal for initiating development. Some of the Ca2+ appears to be released from intracellular stores by the binding of inositol trisphosphate (IP3) to its receptor. However, in sea urchin eggs, other mechanisms appear to participate. Cyclic adenosine diphosphate--ribose (cADPR), a naturally occurring metabolite of nicotinamide adenine dinucleotide, is as potent as IP3 in mobilizing Ca2+ in sea urchin eggs. Experiments with antagonists of the cADPR and IP3 receptors revealed that both Ca2+ mobilizing systems were activated during fertilization. Blockage of either of the systems alone was not sufficient to prevent the sperm-induced Ca2+ transient. This study provides direct evidence for a physiological role of cADPR in the Ca2+ signaling process.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lee, H C -- Aarhus, R -- Walseth, T F -- HD17484/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1993 Jul 16;261(5119):352-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, University of Minnesota, Minneapolis 55455.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8392749" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Diphosphate Ribose/analogs & derivatives/pharmacology
;
Animals
;
Calcium/*metabolism
;
*Calcium Channels
;
Cyclic ADP-Ribose
;
Cyclic AMP/analogs & derivatives/pharmacology
;
Female
;
*Fertilization
;
Heparin/pharmacology
;
Inositol 1,4,5-Trisphosphate/pharmacology
;
Inositol 1,4,5-Trisphosphate Receptors
;
Ovum/*metabolism
;
Receptors, Cell Surface/*physiology
;
*Receptors, Cytoplasmic and Nuclear
;
Sea Urchins
;
Signal Transduction
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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