Publication Date:
2012-02-11
Description:
Long-term memory (LTM) depends on the synthesis of new proteins. Using a temperature-sensitive ribosome-inactivating toxin to acutely inhibit protein synthesis, we screened individual neurons making new proteins after olfactory associative conditioning in Drosophila. Surprisingly, LTM was impaired after inhibiting protein synthesis in two dorsal-anterior-lateral (DAL) neurons but not in the mushroom body (MB), which is considered the adult learning and memory center. Using a photoconvertible fluorescent protein KAEDE to report de novo protein synthesis, we have directly visualized cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB)-dependent transcriptional activation of calcium/calmodulin-dependent protein kinase II and period genes in the DAL neurons after spaced but not massed training. Memory retention was impaired by blocking neural output in DAL during retrieval but not during acquisition or consolidation. These findings suggest an extra-MB memory circuit in Drosophila: LTM consolidation (MB to DAL), storage (DAL), and retrieval (DAL to MB).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, Chun-Chao -- Wu, Jie-Kai -- Lin, Hsuan-Wen -- Pai, Tsung-Pin -- Fu, Tsai-Feng -- Wu, Chia-Lin -- Tully, Tim -- Chiang, Ann-Shyn -- New York, N.Y. -- Science. 2012 Feb 10;335(6069):678-85. doi: 10.1126/science.1212735.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22323813" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Genetically Modified
;
Axons/ultrastructure
;
Brain/cytology/physiology
;
Calcium-Calmodulin-Dependent Protein Kinase Type 2/biosynthesis/genetics
;
Conditioning (Psychology)
;
Cryptochromes/biosynthesis/genetics
;
Cyclic AMP Response Element-Binding Protein/genetics/metabolism
;
Drosophila/cytology/genetics/*physiology
;
Drosophila Proteins/*biosynthesis/genetics/metabolism
;
Gene Expression Profiling
;
Gene Expression Regulation
;
Genes, Insect
;
Luminescent Proteins/biosynthesis/genetics
;
Memory, Long-Term/*physiology
;
Mushroom Bodies/*physiology
;
Neurons/*physiology/ultrastructure
;
Odors
;
Period Circadian Proteins/biosynthesis/genetics
;
Ricin/pharmacology
;
Sensory Receptor Cells/physiology
;
Serine Endopeptidases/biosynthesis/genetics
;
Trans-Activators/genetics/metabolism
;
Transcriptional Activation
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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