Publication Date:
2019-04-11
Description:
Background Mitochondria are the center of energy metabolism and the production of reactive oxygen species (ROS). ROS production results in a burst of “superoxide flashes”, which is always accompanied by depolarization of mitochondrial membrane potential. Superoxide flashes have only been studied in the model plant Arabidopsis thaliana using a complex method to isolate mitochondria. In this study, we present an efficient, easier method to isolate functional mitochondria from floral tissues to measure superoxide flashes. Method We used 0.5 g samples to isolate mitochondria within
Electronic ISSN:
2167-8359
Topics:
Biology
,
Medicine
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