ISSN:
1420-9071
Keywords:
Hymenopteran endoparasites
;
ecdysteroids
;
developmental regulation
;
virus replication
;
polydnaviruses
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract During oviposition the endoparasitic waspCampoletis sonorensis, introduces a polydnavirus into parasitized insects where viral gene expression is required for endoparasite survival. The polydnavirus is integrated into wasp chromosomal DNA and replicates only in the ovary. Ecdysteroids regulate the developmental expression of many insect genes and may regulate polydnavirus replication. Direct verification of viral replication was performed by dot blot hybridization and by amplifying DNA sequences containing the viral integration site; this ‘junction’ fragment cannot be amplified from integrated virus. Thoracic ligations and in vitro ecdysteroid treatments of wasp ovaries support the hypothesis that polydnavirus DNA replication is regulated by ecdysteroid during parasite development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01919157
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