ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Chondroitin sulfate ABC lyases (E.C. 4.2.2.4) are broad-specificity glycosaminoglycan-degrading enzymes. Their preferred substrates are chondroitin sulfate and dermatan sulfate, which are broken down to short oligosaccharides. Proteus vulgaris produces two such lyases, ABC lyase I and II, with molecular weights of 112–113 kDa. Diffraction-quality crystals of both enzymes have been obtained by the hanging-drop vapour-diffusion method. ABC lyase I crystallizes in space group P212121, with unit-cell parameters a = 49.3, b = 95.1, c = 230.0 Å, Z = 4, and diffracts to 1.9 Å resolution. Crystals of ABC lyase II belong to space group P1, with unit-cell parameters a = 64.2, b = 64.3, c = 142.1 Å, α = 95.7, β = 98.1, γ = 95.5°, Z = 2; diffraction extends to at least 2.1 Å.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900005102
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