ISSN:
1365-3059
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
For the production of virus-free patchouli (Pogostemon cablin), isolated meristem tips were cultured on a medium supplemented with 0·2 ppm 6-benzylaminopurine. Multiple shoot proliferation was initiated during the culture. Complete plantlets were regenerated by transferring multiple shoots to a medium devoid of phytohormones. Plantlets thus produced were transplanted to soil with a high rate of survival and tested by enzyme-linked immunosorbent assay (ELISA) to check elimination of patchouli mild mosaic virus. Following further propagation by stem cuttings, virus-free plants were grown in the northern and southern parts of Mindanao Island, Philippines. Significant increases in biomass and essential oil yield were observed in virus-free plants grown at both localities. Using gas chromatographic analyses, a full set of major sesquiterpenes was detected in the essential oils recovered from virus-free patchouli plants. Reinfection of healthy clones with the virus took place within 4 months of cultivation in the field.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3059.1995.tb01673.x
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