ISSN:
1573-5044
Keywords:
Agrobacterium rhizogenes
;
auxin
;
rhizogenesis
;
rolB
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The relative role played in the induction of root differentiation by the morphogenetic generolB, present in the T-DNA ofAgrobacterium rhizogenes, and the specific commitment of different plant cell types, has been evaluated by means of anin vitro-rooting assay. Explants of different origin derived from transgenic tobacco plants transformed by both therolB gene and theGUS reporter gene (E. coli β-glucuronidase) under the transcriptional control ofrolB promoter region were utilized and compared to control transformats containing only therolB-GUS construct. The results obtained demonstrate that: - the pattern of gene expression is similar in both transformants; -rolB-containing tissues are far more effective than untransformed and GUS-transformed controls in regenerating roots; - the presence and expression ofrolB are necessary but not sufficient to trigger rhizogenesis in transformed plant cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02307096
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