ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Autoradiography was found to be a useful method for studying the function of mitochondria. It might provide means to investigate the mitochondrial ATP-ase activity. Applying this method, different problems of metal salt-techniques, firstly the aspecific reactions, can be eliminated. The incubation solution contained 3H-ATP at 100 μc activity. The autoradiographic precipitate seems to appear on the membrane of the mitochondria and in the intracristal space. Atractylate treatment inhibits completely the permeation of 3H-ATP. So-called arreactive mitochondria with retained structure lacking of reaction-precipitate were found besides the mitochondria revealing pronounced activity. This might refer to the manifestation of a functional state. Experiments with P32 ATP may contributs to the more exact determination of original place of the enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00277472
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