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  • 1
    Electronic Resource
    Electronic Resource
    Effect of abscisic acid pretreatments on stomatal reopening in Vicia faba (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 56 (1982), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: After a pretreatment of 2 h exposure to a solution containing 2 × 10−4M ABA, reopening of stomata occurred in epidermal strips of Vicia faba L. cv. Cavalier on an ABA-free incubation solution. After pretreatment with exogenous ABA stomatal apertures were greater when higher levels of KCl were incorporated into the solution used for reopening. Prolonged exposure to exogenous ABA (14 h) did not prevent stomatal reopening upon transfer to ABA-free solutions. However, for both ABA and ABA-free pretreatments, prolonged incubation (1 day after removal of epidermis) resulted in enhanced stomatal apertures when the epidermal strips were exposed to light. This effect was lost 2 days after removal of the epidermis and opening did not occur after 3 days. Epidermal strips containing endogenous ABA were obtained from wilted leaves. Reopening was greatly reduced by the endogenous ABA treatment, and variation of KCl concentration in the incubation solution had little effect on stomatal aperture. It is postulated that during wilting endogenous ABA becomes reversibly bound without loss of activity for a longer period than is obtained using exogenous ABA. The presence of other unidentified compounds may be involved in this process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1982.tb00344.x
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  • 2
    Electronic Resource
    Electronic Resource
    Spherical bodies in the cytoplasm ofCentaurea cyanus L. (Cornflower) cells (1972)
    Springer
    Protoplasma 75 (1972), S. 199-203 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spherical bodies, 650–750 Å in diameter, are present in undifferentiated callus tissue, and in meristematic and differentiated cells in plants ofCentaurea cyanus L. (Cornflower). Most of these organelles are localised in the cytoplasm immediately external to the nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279405
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  • 3
    Electronic Resource
    Electronic Resource
    The induction in vitro of adventitious shoots in Rosa (1988)
    Springer
    Euphytica 37 (1988), S. 31-36 
    Details
    ISSN: 1573-5060
    Keywords: Rosa persica ; Rosa xanthina ; Rosa laevigata ; Rosa wichuraiana ; Rosa rugosa ; Rosa hybrida ; rose ; adventitious shoots ; starch grains ; transplantation ; cellulose plugs ; rose breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Adventitious shoots were formed on excised leaves, roots and callus of Rosa persica x xanthina and on excised leaves of R. laevigata and R. wichuraiana on culture media that included BAP and NAA as growth regulators. Shoots formed freely on freshly cultured callus of R. persica x xanthina but their production declined in successive cultures and ceased after twelve weeks. Transplantation to soil was improved by rooting plantlets in cellulose plugs in vitro and transferring plantlets to soil while still in the plugs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037220
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro procedures for the induction of tetraploidy in a diploid rose (1990)
    Springer
    Euphytica 49 (1990), S. 33-38 
    Details
    ISSN: 1573-5060
    Keywords: Rosa wichuraiana ; rose ; cell cycle ; colcemid ; colchicine ; culture in vitro ; mitotic index ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plantlets of a diploid species, Rosa wichuraiana, were cultured in vitro in liquid medium and treated with spindle inhibitors or tritiated thymidine. Cytological studies were then carried out on cells of root tips which were excised after the treatments. Exposure to 0.05% colchicine gave complete spindle arrest with less toxicity than 0.1% colchicine. Solutions of colcemid gave incomplete spindle arrest even at highly toxic concentrations. After a 0.5 h pulse of 3H thymidine, the periodicity in the curve of percentage of labelled mitoses against time indicated a cell-cycle-time of 10 h. The highest frequency of tetraploid cells was obtained after exposure to 0.05% colchicine for 12 h and it is suggested that colchicine prolonged the cell-cycle-time by 2 h. Seven days after a 12 h exposure to 0.05% colchicine, 32.7% of cells in the root meristem were tetraploid and it is anticipated that a satisfactory yield of non-chimeral tetraploids could be regenerated from such tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024128
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